Nicol A, Garrod D R
J Cell Sci. 1978 Aug;32:377-87. doi: 10.1242/jcs.32.1.377.
Interspecific cell cohesion among 4 species of cellular slime moulds, Dictyostelium discoideum, D. mucoroides, D. purpureum and Polysphondylium violaceum has been studied. Binary mixtures of aggregation-stage cells of the 4 species were shaken in suspension, one species of each pair being labelled with [3H]thymidine. Cell aggregates were sampled at intervals over 24 h and their composition examined by autoradiography. The following results were obtained: (i) Cells of each species were capable of cohesion with those of the other 3 species. (ii) In general cells of both species in any mixture were present in aggregates after 1 h, but were not localized according to species. (iii) Within 8-h aggregates cells of different species were regionally localized, i.e. sorting-out appeared to have taken place. (iv) 24-h aggregates were more varied: in mixtures of Dictyostelium species, the different species were localized within the aggregates; in mixtures of Dictyostelium species with Polsphondylium, there was a tendency for cells of the different species to become segregated into completely separate aggregates. The significance of these results in relation to both previous descriptive work and recent biochemical studies on the mechanism of slime mould cell cohesion is discussed.
对4种细胞黏菌，即盘基网柄菌、黏液网柄菌、紫色网柄菌和紫多孢菌之间的种间细胞黏附进行了研究。将这4种处于聚集阶段的细胞的二元混合物在悬浮液中振荡，每对中的一种细胞用[3H]胸腺嘧啶核苷进行标记。在24小时内每隔一段时间对细胞聚集体进行取样，并通过放射自显影检查其组成。得到了以下结果：（i）每种细胞都能够与其他3种细胞黏附。（ii）一般来说，任何混合物中两种细胞在1小时后都存在于聚集体中，但并未按物种进行定位。（iii）在8小时的聚集体中，不同物种的细胞在区域上进行了定位，即似乎发生了分选。（iv）24小时的聚集体情况更为多样：在盘基网柄菌属物种的混合物中，不同物种在聚集体中进行了定位；在盘基网柄菌属物种与紫多孢菌的混合物中，不同物种的细胞有分离成完全独立聚集体的趋势。讨论了这些结果与先前关于黏菌细胞黏附机制的描述性工作以及最近的生化研究的关系。
