DiNinno V, McCandless E L
Carbohydr Res. 1978 Nov;67(1):235-41. doi: 10.1016/s0008-6215(00)83745-3.
An antibody preparation directed against a structural feature associated with 6-sulphate groups was used to probe structural relations among certain lambda-type carrageenans. Immunochemical and chemical differences are described between the KC1-soluble carrageenans from tetrasporic algal plants of Gigartina corymbifera, Gigartina sp. from San Francisco Bay, Petrocelis middendorfii, Iridaea cordata, Rhodoglossum californicum, and Chondrus crispus. The differences in immunochemical reactivity of the Gigartina and Petrocelis carrageenans relative to the homologous antigen (Chondrus crispus lambda-carrageenan) are attributed to the lower content of 6-sulphate groups on the 4-linked residues in the former carrageenans. Both the immunochemical and chemical data suggest that the Gigartina and Petrocelis carrageenans are largely xi-like in structure but do contain lambda-like features. The i.r. spectrum of the Petrocelis carrageenan differs from that of the Gigartina carrageenans. The carrageenans from I. cordata and R. californicum differ to a lesser degree from Chondrus crispus lambda-type carrageenan. These differences cannot be accounted for by differences in the levels of 6-sulphate groups. Some other structural feature, as yet unidentified, is responsible for the discrepancy in the immunochemical reactivity of these carrageenans to the anti-lambda-carrageenan.
一种针对与6-硫酸根基团相关的结构特征的抗体制剂被用于探究某些λ型角叉菜胶之间的结构关系。描述了来自皱波角叉菜、旧金山湾角叉菜、中肋角叉菜、心形红皮藻、加州红舌藻和皱波角叉菜四分孢子体藻类植物的KCl可溶性角叉菜胶之间的免疫化学和化学差异。相对于同源抗原（皱波角叉菜λ-角叉菜胶），角叉菜和中肋角叉菜胶免疫化学反应性的差异归因于前一种角叉菜胶中4-连接残基上6-硫酸根基团含量较低。免疫化学和化学数据均表明，角叉菜和中肋角叉菜胶在结构上主要类似ξ型，但确实含有λ型特征。中肋角叉菜胶的红外光谱与角叉菜胶的不同。心形红皮藻和加州红舌藻的角叉菜胶与皱波角叉菜λ型角叉菜胶的差异较小。这些差异不能用6-硫酸根基团水平的差异来解释。一些尚未确定的其他结构特征导致了这些角叉菜胶与抗λ-角叉菜胶的免疫化学反应性存在差异。
