DiNinno V, McCandless E L
Carbohydr Res. 1978 Oct;66:85-93. doi: 10.1016/s0008-6215(00)83241-3.
Carrageenans from several species of Eucheuma have been fractionated into KC1-soluble and KC1-insoluble fractions and analyzed by the usual chemical procedures. An anti-kappa-carrageenan, the reactivity of which is directed to kappa-structures (i.e., 3-linked galactose 4-sulphate, and 4-linked 3,6-anhydrogalactose) was used to analyze these carrageenans immunochemically. The antibody preparation shows only a small amount of cross-reactivity with iota-type carrageenans and thus could be used to distinguish kappa- and iota-type carrageenans, the latter having an index of homology of less than 0.2. A comparison of chemical and immunochemical data yielded further information as to the nature of the carrageenan-anti-carrageenan interaction, as well as elucidating the finer structure of carrageenans.
从几种麒麟菜属植物中提取的角叉菜胶已被分离成氯化钾可溶部分和氯化钾不溶部分，并通过常规化学方法进行分析。一种抗κ-角叉菜胶，其反应性针对κ-结构（即3-连接的半乳糖4-硫酸盐和4-连接的3,6-脱水半乳糖），用于对角叉菜胶进行免疫化学分析。该抗体制剂与ι-型角叉菜胶仅表现出少量交叉反应，因此可用于区分κ-型和ι-型角叉菜胶，后者的同源指数小于0.2。化学数据和免疫化学数据的比较产生了关于角叉菜胶-抗角叉菜胶相互作用性质的进一步信息，同时也阐明了角叉菜胶的精细结构。
