染色质的超核小体结构

Supranucleosomal structure of chromatin.

作者信息

Strätling W H, Müller U, Zentgraf H

出版信息

Cell Biol Int Rep. 1978 Sep;2(5):495-9. doi: 10.1016/0309-1651(78)90102-9.

Abstract

Rat liver chromatin was moderately digested by micrococcal nuclease and analysed by centrifugation in isokinetic sucrose gradients and electron microscopy. Two classes of particles sedimenting with about 33S and 60S were characterized. Kinetics of their appearance and disappearance during progressive digestion suggests that they represent monomers and dimers cleaved from a higher order (supranucleosomal) structure of chromatin. Biochemical and electron microscopical results suggest that the monomers and dimers contain eight and sixteen nucleosomes, respectively, which are densely packed into 23 nm (monomer) and 29 nm (dimer) globules.

摘要

用微球菌核酸酶适度消化大鼠肝脏染色质，并通过在等速蔗糖梯度中离心和电子显微镜进行分析。鉴定出了两类沉降系数约为33S和60S的颗粒。在逐步消化过程中它们出现和消失的动力学表明，它们代表从染色质的高阶（超核小体）结构切割下来的单体和二聚体。生化和电子显微镜结果表明，单体和二聚体分别包含八个和十六个核小体，它们紧密堆积成23纳米（单体）和29纳米（二聚体）的球体。