Fukui G M, Berger F M, Chandlee G C, Goldenbaum E G
J Bacteriol. 1968 Oct;96(4):1133-6. doi: 10.1128/jb.96.4.1133-1136.1968.
Chlorphenesin, a simple glycerol ether, when added to Jerne plates greatly reduced the number of hemolytic plaques. This effect appeared to be related to dose, and was clearly demonstrable with antibody-forming spleen cells from mice that had been immunized either with sheep red blood cells or with penicillin G conjugated with Keyhole limpet hemocyanin. Chlorphenesin did not affect the antigen, destroy complement, or interfere with the interaction of complement and the antigen-antibody complexes. Incubation of spleen cell suspensions with chlorphenesin prior to plating was more effective in reducing the number of plaques than was addition of the substance to the plates. It may act by reducing the ability of antibodies to react with antigens or by affecting the release of antibodies from the spleen cells.
氯苯甘醚，一种简单的甘油醚，添加到耶尔恩平板中时可大大减少溶血空斑的数量。这种效应似乎与剂量有关，在用绵羊红细胞或与钥孔血蓝蛋白偶联的青霉素G免疫的小鼠的抗体形成脾细胞中可清楚地证明这一点。氯苯甘醚不影响抗原，不破坏补体，也不干扰补体与抗原-抗体复合物的相互作用。在铺板前将脾细胞悬液与氯苯甘醚一起孵育比将该物质添加到平板中在减少空斑数量方面更有效。它可能通过降低抗体与抗原反应的能力或通过影响抗体从脾细胞中的释放而起作用。
