Rott N N, Bozhkova V P, Kvavilashvili I Sh, Khariton V Iu, Sharova L V
Ontogenez. 1978;9(5):457-69.
The loach blastoderms were isolated from the yolk during the periods of cleavage and blastulation (5--14.5 tau0) and incubated in various isotonic saline media which differed by the content of K+ and Na+. The survival of blastoderms upon the short-term incubation (up to 60 tau0) varied insignificantly in all the media and upon the longer one (up to 120 tau0) was the maximal in the medium with isotonic content of K+ and Na+ and the minimal in the medium with the low content of K+. The cell proliferation upon incubation in the medium with the low content of K+ was inhibited when the blastoderms were isolated at the stage of 8 tau0 and suffered no changes at the stage of 14 tau0. The ability of differentiation manifested itself earlier and the percentage of differentiated embryos was higher in the media with the high content of K+. The content of K+ in the blastoderms isolated at the stage of 7 tau0 changes with its content in the incubation medium; upon isolation at the stages of 8--9 tau0 to blastoderms accumulate K+ irrespective of its content in the medium and at the stage of 10 tau0 maintain the constant level of K+. Hence, the development of isolated blastoderms depends on the content of K+ in the medium and this dependence reduces with the age.
在卵裂期和囊胚期（5-14.5τ0）从卵黄中分离出泥鳅囊胚，并在含有不同K⁺和Na⁺含量的各种等渗盐溶液中培养。在所有培养基中，囊胚短期培养（长达60τ0）后的存活率变化不显著，而在较长时间培养（长达120τ0）后，在K⁺和Na⁺等渗含量的培养基中存活率最高，在K⁺含量低的培养基中存活率最低。当在8τ0阶段分离囊胚时，在K⁺含量低的培养基中培养时细胞增殖受到抑制，而在14τ0阶段分离时则没有变化。在K⁺含量高的培养基中，分化能力出现得更早，分化胚胎的百分比更高。在7τ0阶段分离的囊胚中K⁺的含量随培养培养基中K⁺的含量而变化；在8-9τ0阶段分离时，囊胚积累K⁺，而与培养基中K⁺的含量无关，在10τ0阶段保持K⁺的恒定水平。因此，分离囊胚的发育取决于培养基中K⁺的含量，并且这种依赖性随着年龄的增长而降低。
