Zagris N, Georgatsos J G
Growth. 1984 Winter;48(4):434-44.
Hemoglobin (Hb) synthesis is reduced by 1.5 to 12.4 X 10(-4) M chloramphenicol (CAP) in chick blastoderms at head process and 4-somite stages. Chloramphenicol at 18.6 X 10(-4) M results in decrease of amino acid uptake and incorporation into protein, and inhibits Hb formation completely. However, this effect can be overcome partially when blastoderms are removed from CAP-containing to plain culture medium. The morphological appearance, protein content, and behaviour in amino acid uptake and incorporation of control and CAP-treated blastoderms at the time Hb first makes its appearance can be correlated and seem to reflect changes occurring in the vascular area during the time of blood island organization and subsequent massive Hb synthesis in the simple culture system employed.
在鸡胚头突期和4体节期的胚盘，1.5至12.4×10⁻⁴ M的氯霉素（CAP）可使血红蛋白（Hb）合成减少。18.6×10⁻⁴ M的氯霉素会导致氨基酸摄取减少并抑制其掺入蛋白质，从而完全抑制Hb形成。然而，当将胚盘从含CAP的培养基转移至普通培养基时，这种作用可部分被克服。在Hb首次出现时，对照胚盘和经CAP处理的胚盘的形态外观、蛋白质含量以及氨基酸摄取和掺入行为之间具有相关性，并且似乎反映了在所采用的简单培养系统中血岛形成期间及随后大量Hb合成过程中血管区域发生的变化。
