Wakelin L P, Romanos M, Chen T K, Glaubiger D, Canellakis E S, Waring M J
Biochemistry. 1978 Nov 14;17(23):5057-63. doi: 10.1021/bi00616a031.
An homologous series of diacridines containing two 9-aminoacridine chromophores linked via a simple methylene chain has been studied in order to investigate the minimum interchromophore separation required to permit bifunctional intercalation. Viscometric, sedimentation, and electric dichroism experiments show that compounds having one to four methylene groups in the linker are restricted to monofunctional intercalation, whereas the interaction becomes bifunctional when the chain length is increased to six carbons or more. The results indicate that bifunctional reaction occurs with an interchromophore distance not exceeding 8.8 A, implying that intercalation by these compounds is not subject to neighbor exclusion if the mode of binding is of the classical intercalation type.
为了研究允许双功能嵌入所需的发色团间最小间距，对一系列通过简单亚甲基链连接两个9-氨基吖啶发色团的二吖啶同系物进行了研究。粘度测定、沉降和电二色性实验表明，连接基中含有一至四个亚甲基的化合物仅限于单功能嵌入，而当链长增加到六个碳或更长时，相互作用变为双功能。结果表明，双功能反应发生时发色团间距离不超过8.8 Å，这意味着如果结合模式为经典嵌入类型，这些化合物的嵌入不受邻位排斥的影响。
