有丝分裂器：用弱酸对蛋白质进行选择性提取。
Mitotic apparatus: the selective extraction of protein with mild acid.
作者信息
Bibring T, Baxandall J
出版信息
Science. 1968 Jul 26;161(3839):377-9. doi: 10.1126/science.161.3839.377.
PMID:5690797
Abstract
The treatment of isolated mitotic apparatus with mild (pH 3) hydrochloric acid results in the extraction of less than 10 percent of its protein, accompanied by the selective morphological disappearance of the microtubules. The same extraction can be shown to dissolve outer doublet microtubules from sperm flagella. A protein with points of similarity to the flagellar microtubule protein is the major component of the extract from mitotic apparatus.
摘要
用温和的（pH 3）盐酸处理分离出的有丝分裂器，结果显示其蛋白质的提取量不到10%，同时微管会出现选择性的形态消失。同样的提取过程也能溶解精子鞭毛中的外双联微管。一种与鞭毛微管蛋白有相似之处的蛋白质是有丝分裂器提取物的主要成分。
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引用本文的文献
1
Selective extraction of isolated mitotic apparatus. Evidence that typical microtubule protein is extracted by organic mercurial.分离有丝分裂器的选择性提取。有证据表明典型的微管蛋白可被有机汞提取。
J Cell Biol. 1971 Feb;48(2):324-39. doi: 10.1083/jcb.48.2.324.
2
Immunochemical studies of 22S protein from isolated mitotic apparatus.对分离出的有丝分裂器中22S蛋白质的免疫化学研究。
J Cell Biol. 1969 May;41(2):577-90. doi: 10.1083/jcb.41.2.577.
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