对分离出的有丝分裂器中22S蛋白质的免疫化学研究。
Immunochemical studies of 22S protein from isolated mitotic apparatus.
作者信息
Bibring T, Baxandall J
出版信息
J Cell Biol. 1969 May;41(2):577-90. doi: 10.1083/jcb.41.2.577.
Abstract
Evidence is presented that the "22S protein" of mitotic apparatus isolated from sea urchin eggs is not microtubule protein. An antibody preparation active against 22S protein is described, and immunochemical studies of the distribution of 22S protein in various cellular fractions and among morphological features of mitotic apparatus are reported. The protein is ubiquitous in the metaphase egg fractions that were tested but is not found in sperm flagella. It is immunologically distinct from proposed microtubule protein isolated from mitotic apparatus by the method of Sakai, and from proposed microtubule protein obtained after extraction with mild acid. It exists in nontubule material of isolated mitotic apparatus but is not detectable in microtubules.
摘要
有证据表明，从海胆卵中分离出的有丝分裂器的“22S蛋白”不是微管蛋白。描述了一种对22S蛋白有活性的抗体制剂，并报道了对22S蛋白在各种细胞组分以及有丝分裂器形态特征中的分布进行的免疫化学研究。该蛋白在所测试的中期卵组分中普遍存在，但在精子鞭毛中未发现。它在免疫学上与通过酒井方法从有丝分裂器中分离出的拟微管蛋白以及用弱酸提取后得到的拟微管蛋白不同。它存在于分离出的有丝分裂器的非微管物质中，但在微管中无法检测到。
相似文献
1
Immunochemical studies of 22S protein from isolated mitotic apparatus.对分离出的有丝分裂器中22S蛋白质的免疫化学研究。
J Cell Biol. 1969 May;41(2):577-90. doi: 10.1083/jcb.41.2.577.
2
Purification of the mitotic apparatus protein of sea urchin eggs.海胆卵有丝分裂器蛋白的纯化
Exp Cell Res. 1968 Apr;50(1):54-64. doi: 10.1016/0014-4827(68)90393-5.
3
Mitotic apparatus: the selective extraction of protein with mild acid.有丝分裂器：用弱酸对蛋白质进行选择性提取。
Science. 1968 Jul 26;161(3839):377-9. doi: 10.1126/science.161.3839.377.
4
Comparative study of proteins from sea urchin sperm flagella and unfertilized eggs.海胆精子鞭毛和未受精卵蛋白质的比较研究。
J Mol Biol. 1968 Dec;38(3):427-9. doi: 10.1016/0022-2836(68)90397-5.
5
A Ca-activated ATPase in the mitotic apparatus of the sea urchin egg (isolated by a new method).海胆卵有丝分裂器中的一种钙激活ATP酶（通过一种新方法分离得到）。
Exp Cell Res. 1972 Feb;70(2):325-32. doi: 10.1016/0014-4827(72)90143-7.
6
Further evidence of the similarity of microtubule protein from mitotic apparatus and sperm tail of the sea urchin, as a substrate in thiol-disulfide exchange reaction.海胆有丝分裂器和精子尾部的微管蛋白作为硫醇-二硫键交换反应底物的相似性的进一步证据。
Exp Cell Res. 1973 Jun;79(2):445-6. doi: 10.1016/0014-4827(73)90464-3.
7
Serological similarity of flagellar and mitotic microtubules.鞭毛微管和有丝分裂微管的血清学相似性。
J Cell Biol. 1971 Sep;50(3):762-73. doi: 10.1083/jcb.50.3.762.
8
The mitotic apparatus. Identification of the major soluble component of the glycol-isolated mitotic apparatus.有丝分裂器。糖酵解分离的有丝分裂器主要可溶性成分的鉴定。
J Cell Biol. 1967 Feb;32(2):243-53. doi: 10.1083/jcb.32.2.243.
9
A comparative study of the isolation of the cortex and the role of the calcium-insoluble protein in several species of sea urchin egg.几种海胆卵皮质分离及钙不溶性蛋白作用的比较研究。
J Cell Biol. 1969 Apr;41(1):133-44. doi: 10.1083/jcb.41.1.133.
10
An estimate of the amount of microtubule protein in the isolated mitotic apparatus.对分离出的有丝分裂器中微管蛋白含量的一种估计。
J Cell Sci. 1970 Jan;6(1):159-76. doi: 10.1242/jcs.6.1.159.
引用本文的文献
1
Selective extraction of isolated mitotic apparatus. Evidence that typical microtubule protein is extracted by organic mercurial.分离有丝分裂器的选择性提取。有证据表明典型的微管蛋白可被有机汞提取。
J Cell Biol. 1971 Feb;48(2):324-39. doi: 10.1083/jcb.48.2.324.
2
A functional mitotic spindle prepared from mammalian cells in culture.从培养的哺乳动物细胞中制备的功能性有丝分裂纺锤体。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1559-63. doi: 10.1073/pnas.71.4.1559.
3
Electron microscope studies on puromycin-induced changes in nerve cells of the medulla oblongata.关于嘌呤霉素诱导延髓神经细胞变化的电子显微镜研究。
J Cell Biol. 1974 Dec;63(3):1091-7. doi: 10.1083/jcb.63.3.1091.
4
Serological similarity of flagellar and mitotic microtubules.鞭毛微管和有丝分裂微管的血清学相似性。
J Cell Biol. 1971 Sep;50(3):762-73. doi: 10.1083/jcb.50.3.762.
5
Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.微管蛋白α亚基的异质性以及单个生物体中微管蛋白的变异性。
J Cell Biol. 1976 May;69(2):301-12. doi: 10.1083/jcb.69.2.301.
本文引用的文献
1
The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.分裂细胞有丝分裂器的分离与生化特性分析
Proc Natl Acad Sci U S A. 1952 Sep;38(9):826-38. doi: 10.1073/pnas.38.9.826.
2
THE MITOTIC APPARATUS. PHYSICAL-CHEMICAL FACTORS CONTROLLING STABILITY.有丝分裂器。控制稳定性的物理化学因素。
J Cell Biol. 1965 Apr;25(1):SUPPL:137-44. doi: 10.1083/jcb.25.1.137.
3
A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.一种用于电子显微镜的简化柠檬酸铅染色法。
J Cell Biol. 1965 May;25(2):407-8. doi: 10.1083/jcb.25.2.407.
4
THE ISOLATION AND CHARACTERIZATION OF ASTERS FROM ARTIFICIALLY ACTIVATED SEA URCHIN EGGS.从人工激活的海胆卵中分离并鉴定星体。
Exp Cell Res. 1964 Nov;36:256-69. doi: 10.1016/0014-4827(64)90206-x.
5
IMMUNO-ELECTRON MICROSCOPE ANALYSIS OF THE SURFACE LAYERS OF THE UNFERTILISED SEA URCHIN EGG. II. LOCALISATION OF SURFACE ANTIGENS.未受精海胆卵表面层的免疫电子显微镜分析。II. 表面抗原的定位
J Cell Biol. 1964 Dec;23(3):629-50. doi: 10.1083/jcb.23.3.629.
6
PERCENTAGE INCORPORATION OF LEUCINE LABELLED WITH CARBON-14 INTO ISOLATED MITOTIC APPARATUS DURING EARLY DEVELOPMENT OF SEA URCHIN EGGS.在海胆卵早期发育过程中，碳-14标记的亮氨酸掺入分离的有丝分裂器的百分比。
Nature. 1964 Nov 21;204:805-7. doi: 10.1038/204805a0.
7
THE ACUTE TOXICITY OF CRYSTALLIZED FERRITIN, CADMIUM-FREE FERRITIN AND CDCL2 ON EHRLICH ASCITES CARCINOMA CELLS.结晶铁蛋白、无镉铁蛋白和氯化镉对艾氏腹水癌细胞的急性毒性
Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 May 11;248:153-65. doi: 10.1007/BF00246670.
8
STUDIES ON THE PROTEIN COMPONENTS OF CILIA FROM TETRAHYMENA PYRIFORMIS.梨形四膜虫纤毛蛋白质成分的研究
Proc Natl Acad Sci U S A. 1963 Nov;50(5):1002-10. doi: 10.1073/pnas.50.5.1002.
9
The mitotic apparatus. Fine structure of the isolated unit.有丝分裂器。分离单元的精细结构。
J Cell Biol. 1962 Nov;15(2):279-87. doi: 10.1083/jcb.15.2.279.
10
A two-layer technique for detecting surface antigens in the sea urchin egg with ferritin-conjugated antibody.一种用铁蛋白偶联抗体检测海胆卵表面抗原的双层技术。
J Cell Biol. 1962 Jul;14(1):144-51. doi: 10.1083/jcb.14.1.144.
Zotero 插件