目标细胞核中无类固醇的“活化”雌激素受体的出现。

The occurrence of steroid-free, "activated" estrogen receptor in target cell nuclei.

作者信息

Jungblut P W, Kallweit E, Sierralta W, Truitt A J, Wagner R K

出版信息

Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1259-68. doi: 10.1515/bchm2.1978.359.2.1259.

DOI:10.1515/bchm2.1978.359.2.1259

Abstract

New techniques for the adrenalectomy of pigs and for the isolation of pig uterus nuclei are described. The isolated nuclei were analysed for their content of estradiol and of estradiol receptor. The concentration of the latter exceeded that of the hormone in extracts of uterine nuclei from ovariectomized pigs by ratios of 1.8--10.3. Substantial amounts of both monomer and "activated" dimer receptor but no estradiol at all were extracted from uterine nuclei of ovariectomized/adrenalectomized pigs. The mechanism of action of steroid hormones is discussed on the basis of these results.

摘要

本文描述了猪肾上腺切除术及猪子宫细胞核分离的新技术。对分离出的细胞核进行了雌二醇及其受体含量的分析。在去卵巢猪子宫细胞核提取物中，后者（雌二醇受体）与激素的浓度比为1.8至10.3，前者浓度超过后者。从去卵巢/肾上腺切除猪的子宫细胞核中提取出了大量的单体和“活化”二聚体受体，但根本没有雌二醇。基于这些结果对类固醇激素的作用机制进行了讨论。