Kalimi M, Fujimoto G I
Biochim Biophys Acta. 1978 Jan 18;538(2):231-5. doi: 10.1016/0304-4165(78)90350-1.
Colchicine has previously been shown in our laboratory to inhibit 17 beta-estradiol stimulation of uterine water uptake in the immature rat measured 6 h after administration of the agents. We sought to determine whether this effect was mediated through colchicine action on translocation of estradiol receptor complex into the uterine cell nucleus. The time course of estradiol effect on uterine water uptake was followed with and without concurrent colchicine administration up to 6 h after administration. At no time during this period did there appear to be any influence of colchicine on translocation of the estradiol receptor complex into the nucleus. Examination of physical chemical characteristics of the nuclear estradiol receptr complex after estradiol and estradiol plus colchicine treatments revealed no observable differences. Thus, colchicine inhibition of estradiol-stimulated uterine water retention does not appear to be mediated through inhibition of nuclear translocation of estradiol-receptor complex nor to be due to any reduced retention time of estradiol-receptor complex in uterine nuclei.
在我们实验室之前的研究中已表明，秋水仙碱可抑制未成熟大鼠子宫对水的摄取，这种抑制作用是在给予药物6小时后进行测量的，该摄取过程由17β-雌二醇刺激引起。我们试图确定这种效应是否是通过秋水仙碱对雌二醇受体复合物转位进入子宫细胞核的作用来介导的。在给药后长达6小时的时间内，我们观察了在同时给予和不给予秋水仙碱的情况下，雌二醇对子宫水摄取的影响的时间进程。在此期间的任何时候，秋水仙碱似乎都未对雌二醇受体复合物转位进入细胞核产生任何影响。对雌二醇和雌二醇加秋水仙碱处理后的细胞核雌二醇受体复合物的物理化学特性进行检查，未发现可观察到的差异。因此，秋水仙碱对雌二醇刺激的子宫水潴留的抑制作用似乎不是通过抑制雌二醇受体复合物的核转位介导的，也不是由于雌二醇受体复合物在子宫细胞核中的保留时间缩短所致。
