McAteer J A, Hegre O D
In Vitro. 1978 Sep;14(9):795-803. doi: 10.1007/BF02617974.
A method of perfusion organ culture is described in which explants cultured at the air-medium interface are bathed by a continuous flow of nutrient medium. Morphological studies on the fetal rat lung indicate that explant development in this system is comparable to that obtained using standard organ-culture dishes. Medium supply is easily manipulated and continuous sampling of the effluent stream is possible without disturbing the immediate explant environment. The basic design facilitates secretory-response studies on cultured organ explants as demonstrated by a study of glucose-stimulated insulin release by the neonatal rat pancreas.
本文描述了一种灌注器官培养方法，在该方法中，培养于气-培养基界面的外植体被连续流动的营养培养基所浸润。对胎鼠肺的形态学研究表明，该系统中外植体的发育与使用标准器官培养皿所获得的发育情况相当。培养基供应易于操控，并且可以对流出液流进行连续采样，而不会干扰外植体的直接环境。如对新生大鼠胰腺葡萄糖刺激的胰岛素释放的研究所证明的那样，这种基本设计便于对培养的器官外植体进行分泌反应研究。
