Průchová J, Vrána M, Sladký K
J Hyg Epidemiol Microbiol Immunol. 1978;22(1):47-52.
In the present study, the authors verified earlier experience with the rat-paw-oedema test and tried to improve the method and to increase its sensitivity so as to make possible, by means of this test, to screen vaccines producing different reactivity. Toxic and non-toxic B. pertussis suspensions were used as test material. The sensitivity of the animal to the suspension was increased by sensitization of the rats using Alditepera and by influencing the pituitary using different doses of Hydrocortisone. Influencing the pituitary by means of cortisone was found to be more suitable for screening assessment of the reactivity to suspension than sensitizing the animals.
在本研究中，作者验证了此前大鼠足爪水肿试验的经验，并试图改进该方法并提高其灵敏度，以便通过该试验筛选出产生不同反应性的疫苗。使用有毒和无毒的百日咳杆菌悬液作为试验材料。通过使用醛狄特帕致敏大鼠以及使用不同剂量的氢化可的松影响垂体，提高了动物对悬液的敏感性。结果发现，通过可的松影响垂体比使动物致敏更适合用于筛选评估对悬液的反应性。
