Manclark C R, Urban M A, Summers E P, Vickers J H
J Med Microbiol. 1977 Feb;10(1):115-20. doi: 10.1099/00222615-10-1-115.
A method is described of using the production of oedema in the foot pad of rats as an index of the ability of pertussis vaccines to cause local reactions. At a suitable time after the subcutaneous injection of the vaccine into hind paw of the rat, the foot is excised and weighed. The technique is reproducible and most useful for the detection of oedema produced by pertussis-vaccine components sensitive to heating at 80 degrees C for 30 min. Substances in pertussis vaccine that produce rat-paw oedema gave maximal reactions at 4 and 12 h. but were best differentiated 9 h after injection.
本文描述了一种利用大鼠足垫水肿的产生作为百日咳疫苗引起局部反应能力指标的方法。在将疫苗皮下注射到大鼠后爪的适当时间，切除足部并称重。该技术具有可重复性，对于检测对80℃加热30分钟敏感的百日咳疫苗成分产生的水肿最为有用。百日咳疫苗中产生大鼠爪水肿的物质在4小时和12小时时产生最大反应，但在注射后9小时时最易区分。
