Carić-Lazar M, Schwarz R T, Scholtissek C
Eur J Biochem. 1978 Nov 15;91(2):351-61. doi: 10.1111/j.1432-1033.1978.tb12687.x.
The influence of infection with three different lipid-containing RNA viruses, Newcastle disease virus, fowl plague virus, and Semliki Forest virus on the phosphatidylcholine precursors of chick embryo cells and of baby hamster kidney (BHK) cells has been measured. In chick embryo cells infection with Newcastle disease virus does not influence the energy charge, or the distribution and absolute pool sizes of the precursors or the choline phosphotransferase activity. In chick embryo cells infected with fowl plague virus the CDP-choline pool increases because of an inhibition of the choline phosphotransferase activity. The phosphorylcholine and CTP pools are smaller in infected cells when compared with mock-infected ones, although the energy charge is not influenced by infection. In chick embryo cells as well as in BHK cells the energy charge is diminished by infection with Semliki Forest virus. Therefore the CTP and phosphorylcholine pools are decreased. The CDP-choline pool in chick embryo cells becomes extremely small after infection with Semliki Forest virus because of a significant stimulation of the choline phosphotransferase. In BHK cells infected with Semliki Forest virus the opposite effect is observed. There are also severe effects on the uptake of the labeled precursors by infection. One and the same virus (Semliki Forest virus) has two completely different effects on the phosphatidylcholine precursors when infecting two different cell types. If one and the same cell type (chick embryo cells) is infected with three different lipid-containing RNA viruses also completely different effects on the phosphatidylcholine precursors were observed. Thus, each virus develops its own strategy to influence the lipid metabolism of the host cell, depending also on the choice of the host. This explains the many disturbing contradictory results described in the literature about the influence of lipid-containing viruses on the lipid metabolism of the host.
已测定三种不同的含脂质RNA病毒，即新城疫病毒、禽瘟病毒和Semliki森林病毒感染对鸡胚细胞和幼仓鼠肾（BHK）细胞磷脂酰胆碱前体的影响。在鸡胚细胞中，新城疫病毒感染不影响能荷、前体的分布和绝对库大小或胆碱磷酸转移酶活性。在感染禽瘟病毒的鸡胚细胞中，由于胆碱磷酸转移酶活性受到抑制，CDP-胆碱库增加。与 mock 感染的细胞相比，感染细胞中的磷酸胆碱和CTP库较小，尽管感染不影响能荷。在鸡胚细胞和BHK细胞中，Semliki森林病毒感染都会降低能荷。因此，CTP和磷酸胆碱库减少。感染Semliki森林病毒后，鸡胚细胞中的CDP-胆碱库变得极小，这是因为胆碱磷酸转移酶受到显著刺激。在感染Semliki森林病毒的BHK细胞中，观察到相反的效果。感染对标记前体的摄取也有严重影响。同一种病毒（Semliki森林病毒）在感染两种不同细胞类型时，对磷脂酰胆碱前体有两种完全不同的影响。如果用三种不同的含脂质RNA病毒感染同一种细胞类型（鸡胚细胞），对磷脂酰胆碱前体也会观察到完全不同的效果。因此，每种病毒都会制定自己的策略来影响宿主细胞的脂质代谢，这也取决于宿主的选择。这就解释了文献中关于含脂质病毒对宿主脂质代谢影响的许多令人困惑的矛盾结果。
