Schwarz R T, Rohrschneider J M, Schmidt M F
J Virol. 1976 Sep;19(3):782-91. doi: 10.1128/JVI.19.3.782-791.1976.
Tunicamycin, a new antibiotic, halts the formation of physical particles of Semliki forest and fowl plague virus, whereas avian oncornavirus particles which show a reduction in infectivity and do not contain detectable labeled glycoprotein are released in the presence of the drug. In Semliki forest virus-infected cells only the protein moieties of the glycoproteins could be labeled. In cells infected with fowl plague and avian sarcoma virus neither intact glycoproteins nor their protein moieties could be detected. By using a protease inhibitor (N-alpha-p-tosyl-L-lysin chloromethyl ketone, TLCK) it could be shown, however, that the carbohydrate-free hemagglutinin precursor of influenza virus is synthesized but is presumably degraded by intracellular proteases in the absence of TLCK as a consequence of the lack of carbohydrate.
衣霉素是一种新型抗生素，它能阻止塞姆利基森林病毒和禽瘟病毒物理颗粒的形成，而禽肿瘤病毒颗粒在该药物存在的情况下仍会释放，但其感染性降低且不含可检测到的标记糖蛋白。在感染塞姆利基森林病毒的细胞中，只有糖蛋白的蛋白质部分能够被标记。在感染禽瘟病毒和禽肉瘤病毒的细胞中，既检测不到完整的糖蛋白，也检测不到其蛋白质部分。然而，通过使用一种蛋白酶抑制剂（N-α-对甲苯磺酰-L-赖氨酸氯甲基酮，TLCK）可以表明，流感病毒无糖的血凝素前体是合成的，但由于缺乏碳水化合物，在没有TLCK的情况下，它可能会被细胞内蛋白酶降解。
