Dyer R G, Mayes L C
Exp Brain Res. 1978 Nov 15;33(3-4):583-92. doi: 10.1007/BF00235576.
The experiments were designed to specifiy optimal parameters of hypothalamic electrical stimulation to induce ovulation in rats anaesthetised with pentobarbitone during the "critical period" on the afternoon of pro-oestrous. We have established that duration of stimulation in the arcuate region of the hypothalamus is of greater importance than total number of pulses delivered. The minimum period of stimulation to ensure that all rats ovulated was 45 min. However, stimulation in the arcuate region did not have to be continuous throughout this period. Indeed, three 5-min bursts at 100 Hz distributed equally through 45 min always caused ovulation whereas a continuous 15-min stimulation at the same frequency was without effect. With 30 min of continuous stimulation in the arcuate region, at frequencies ranging from 10 to 100 Hz, approximately half of the rats ovulated.
这些实验旨在确定在动情前期下午的“关键期”，对用戊巴比妥麻醉的大鼠进行下丘脑电刺激以诱导排卵的最佳参数。我们已经确定，下丘脑弓状区域的刺激持续时间比传递的脉冲总数更为重要。确保所有大鼠排卵的最短刺激时间为45分钟。然而，在此期间，弓状区域的刺激不必持续。实际上，在45分钟内均匀分布的三个100赫兹、每次5分钟的脉冲串总能引起排卵，而以相同频率持续15分钟的刺激则无效。在弓状区域进行30分钟的持续刺激，频率范围为10至100赫兹时，约一半的大鼠排卵。
