Colombo J A
Brain Res. 1978 Apr 14;144(2):313-23. doi: 10.1016/0006-8993(78)90157-9.
In acute experiments under pentobarbital anesthesia, the brains of adult male rats were stimulated bilaterally with trains of monophasic pulses at different frequencies and current intensities through bipolar, concentric, stainless steel electrodes. Stimulation of the POA-SQ region at 3, 10 (200 muA) or 200 Hz (100 muA) increased plasma prolactin levels within 30 min of the onset of stimulation. Stimulus parameters fully effective when applied to the POA-SQ or ARC-MED. EM. regions were ineffective when applied to the ARC-VMH region. Maximal effects were obtained with 10 Hz (200 muA) stimuli applied to the POA-SQ region. LH levels were increased after stimulation of any of the three regions--POA-SQ, ARC-VMH or ARC-MED. EM. Conversely to the prolactin response, LH levels did not rise after 3 Hz stimulation of the POA-SQ region. In male rats under pentobarbital anesthesia, LH levels seemed to be affected maximally with a stimulus frequency rate of about 50 Hz applied to the POA-SQ region. The results suggest a potential physiological role of frequency discrimination in the functional segregation of LH and prolactin release controls.
在戊巴比妥麻醉下的急性实验中，通过双极、同心不锈钢电极，以不同频率和电流强度的单相脉冲串对成年雄性大鼠的大脑进行双侧刺激。在刺激开始后30分钟内，以3、10（200微安）或200赫兹（100微安）刺激视前区-下丘脑前部（POA-SQ）区域可提高血浆催乳素水平。当应用于POA-SQ或弓状核-内侧视前区（ARC-MED. EM.）区域时，刺激参数完全有效；而当应用于弓状核-腹内侧核（ARC-VMH）区域时则无效。以10赫兹（200微安）刺激POA-SQ区域可获得最大效应。刺激三个区域中的任何一个——POA-SQ、ARC-VMH或ARC-MED. EM.后，促黄体生成素（LH）水平都会升高。与催乳素反应相反，以3赫兹刺激POA-SQ区域后，LH水平并未升高。在戊巴比妥麻醉的雄性大鼠中，当以约50赫兹的刺激频率刺激POA-SQ区域时，LH水平似乎受到的影响最大。结果表明频率辨别在LH和催乳素释放控制的功能分离中可能具有生理作用。
