Hayez-Delatte F, Feremans W
Bull Cancer. 1975 Jan-Mar;62(1):29-36.
The authors tried to determine a dose-effect relation between the concentration of Bleomycin and the number of chromosomal aberrations "in vitro" in human lymphocytes during 48 h cultures. Concentrations of 1 gamma/ml, 2 gamma/ml, 10 gamma/ml and 20 gamma/ml were used. The number of normal cells seems to decrease according an exponential function; at a concentration of 20 gamma/ml, 15 p. 100 of the cells were apparently normal and this rises the problem of chemotherapeutic agent resistance. The number of one hit and two hit damages increases according to the dose of Bleomycine but it was not possible to determine a mathematic relation of the phenomenon. Like viruses and others chemicals, Bleomycin was responsible for "pulverisation" of chromosomes in several cells.
作者试图确定博来霉素浓度与人类淋巴细胞在48小时培养期间“体外”染色体畸变数量之间的剂量效应关系。使用了1微克/毫升、2微克/毫升、10微克/毫升和20微克/毫升的浓度。正常细胞数量似乎呈指数函数减少；在20微克/毫升的浓度下，15%的细胞明显正常，这就引发了化疗药物耐药性的问题。一次打击和两次打击损伤的数量随博来霉素剂量增加，但无法确定该现象的数学关系。与病毒和其他化学物质一样，博来霉素导致了几个细胞中的染色体“粉碎”。
