Geller B D, Speirs R S
Immunology. 1968 Nov;15(5):707-16.
A single dose of 15 μg (0·6 mg/kg) Actinomycin-D administered to female BDF mice after intraperitoneal injections of pertussis vaccine and tetanus toxoid caused: (1) a prolonged depression in thymic and splenic weights; (2) an inhibition of the bone marrow leucocytosis which normally occurs between the 2nd and 14th days after antigen injection; (3) a blood leucopenia and marked decrease in the number of mononuclear cells in the inflammatory exudate during both the primary response and a secondary response initiated 4 weeks later; and (4) an inhibition of primary immunization, as evidenced by low antitoxin titres 4 weeks after priming, and by the failure to achieve a normal secondary response to a challenging injection of antigen given 4 weeks later. It was suggested that the sensitivity of mononuclear cells to Actinomycin-D may be responsible for the suppression of priming, possibly by preventing RNA synthesis and differentiation induced by antigen.
在对雌性BDF小鼠腹腔注射百日咳疫苗和破伤风类毒素后，给予单剂量15μg（0.6mg/kg）放线菌素-D，结果导致：（1）胸腺和脾脏重量长期降低；（2）抑制抗原注射后第2天至第14天通常出现的骨髓白细胞增多；（3）在初次反应以及4周后引发的二次反应期间，出现血白细胞减少以及炎性渗出物中单核细胞数量显著减少；（4）抑制初次免疫，这表现为初次免疫4周后抗毒素滴度较低，以及4周后给予挑战性抗原注射时未能产生正常的二次反应。有人提出，单核细胞对放线菌素-D的敏感性可能是引发抑制的原因，可能是通过阻止抗原诱导的RNA合成和分化。
