BUTLER W T, COONS A H
J Exp Med. 1964 Dec 1;120(6):1051-60. doi: 10.1084/jem.120.6.1051.
The effect of drugs upon the primary and the secondary antibody response to diphtheria toxoid in mice was studied using an experimental system previously described. Triethylenethiophosphoramide (thio-TEPA), chloramphenicol, 6-mercaptopurine, 8-azaguanine, and versenate were found to inhibit, partially or completely,"priming" for the secondary response. Thio-TEPA, chloramphenicol, and 6-mercaptopurine, in doses exceeding those effective in inhibiting priming, did not cause alteration of the secondary response when given only during the secondary response. However, when chloramphenicol and amethopterin were given for 5 days prior to and at least 5 days after the second antigen injection, slight suppression of peak secondary titers occurred. Therefore, drug dosages effective in suppressing priming had less effect on the secondary response. It thus appears that there is a real difference between "priming" and the induction of antibody synthesis.
利用先前描述的实验系统,研究了药物对小鼠对白喉类毒素的初次和二次抗体反应的影响。发现三乙烯硫代磷酰胺(硫替派)、氯霉素、6-巯基嘌呤、8-氮杂鸟嘌呤和依地酸盐可部分或完全抑制二次反应的“致敏”。硫替派、氯霉素和6-巯基嘌呤,当给予剂量超过有效抑制致敏的剂量时,仅在二次反应期间给予不会引起二次反应的改变。然而,当在第二次抗原注射前5天和至少第二次抗原注射后5天给予氯霉素和氨甲蝶呤时,二次滴度峰值出现轻微抑制。因此,有效抑制致敏的药物剂量对二次反应的影响较小。由此看来,“致敏”和抗体合成的诱导之间存在真正的差异。