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改良比色超微量法测定血清中游离脂肪酸

Modified colorimetric ultramicro method for estimating Nefa in serum.

作者信息

Elphick M C

出版信息

J Clin Pathol. 1968 Sep;21(5):567-70. doi: 10.1136/jcp.21.5.567.

Abstract

A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 mul samples; it is thus suitable for work on newborn babies and small laboratory animals.

摘要

本文描述了一种基于诺瓦克超微量法估算血清游离脂肪酸的方法。该方法克服了获取准确标准曲线的困难,并减少了磷脂的干扰作用。该方法操作简单,在50微升样本中能给出准确、精确的结果;因此适用于新生儿和小型实验动物的研究。

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