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1
Modified colorimetric ultramicro method for estimating Nefa in serum.改良比色超微量法测定血清中游离脂肪酸
J Clin Pathol. 1968 Sep;21(5):567-70. doi: 10.1136/jcp.21.5.567.
2
Automated modification of Duncombe's method for the ultramicro determination of serum free fatty acids.
J Lipid Res. 1975 Sep;16(5):402-6.
3
A new colorimetric micro-determination of free fatty acids in serum.血清中游离脂肪酸的一种新的比色微量测定法。
Clin Chim Acta. 1973 Feb 12;43(3):317-20. doi: 10.1016/0009-8981(73)90468-3.
4
[Evaluation of colorimetric serum free fatty acid determination methods. 2. Elphick's method].
Rinsho Byori. 1970 Feb;18(2):124-6.
5
[Evaluation of colorimetric determination of serum free fatty acids. 6. Influence of coexisting substances in the serum (especially phospholipids and bilirubin].
Rinsho Byori. 1970 Oct;18(10):719-22.
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A more sensitive and stable colorimetric determination of free fatty acids in blood.
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Colorimetric method for free fatty acids in serum validated by comparison with gas chromatography.
Clin Chem. 1981 Jun;27(6):924-6.
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[Evaluation of colorimetric serum free fatty acid determination method. 3. Comparative study of the color reagents].
Rinsho Byori. 1970 Jun;18(6):451-4.
9
An accurate, sensitive and reproducible method for the colorimetric estimation of free fatty acids in plasma.
Onderstepoort J Vet Res. 1977 Sep;44(3):169-72.
10
Free fatty acid estimation by a semi-automated fluorimetric method.
Clin Chim Acta. 1973 Dec 27;49(3):341-8. doi: 10.1016/0009-8981(73)90231-3.

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本文引用的文献

1
COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS IN BIOLOGICAL FLUIDS.生物体液中游离脂肪酸的比色测定法。
J Lipid Res. 1965 Jan;6:16-20.
2
PHOTOMETRIC ADAPTATION OF DOLE'S MICRODETERMINATION OF FREE FATTY ACIDS.多尔微量法测定游离脂肪酸的光度适应
J Lipid Res. 1965 Jan;6:157-9.
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A colorimetric method for the assay of some higher fatty acids in blood and serum.一种用于测定血液和血清中某些高级脂肪酸的比色法。
Clin Chim Acta. 1961 Nov;6:887-9. doi: 10.1016/0009-8981(61)90180-2.
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Titration of free fatty acids of plasma: a study of current methods and a new modification.血浆游离脂肪酸的滴定：当前方法研究及一种新改良方法
J Lipid Res. 1960 Apr;1:199-202.
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Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
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Unesterified fatty acid in human blood plasma. II. The transport function of unesterified fatty acid.人体血浆中的游离脂肪酸。II. 游离脂肪酸的转运功能。
J Clin Invest. 1957 Jun;36(6 Part 1):810-5. doi: 10.1172/JCI103486.
7
A relation between non-esterified fatty acids in plasma and the metabolism of glucose.血浆中非酯化脂肪酸与葡萄糖代谢之间的关系。
J Clin Invest. 1956 Feb;35(2):150-4. doi: 10.1172/JCI103259.

改良比色超微量法测定血清中游离脂肪酸

Modified colorimetric ultramicro method for estimating Nefa in serum.

作者信息

Elphick M C

出版信息

J Clin Pathol. 1968 Sep;21(5):567-70. doi: 10.1136/jcp.21.5.567.

DOI:10.1136/jcp.21.5.567
PMID:5697360
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC473863/
Abstract

A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 mul samples; it is thus suitable for work on newborn babies and small laboratory animals.

摘要

本文描述了一种基于诺瓦克超微量法估算血清游离脂肪酸的方法。该方法克服了获取准确标准曲线的困难，并减少了磷脂的干扰作用。该方法操作简单，在50微升样本中能给出准确、精确的结果；因此适用于新生儿和小型实验动物的研究。