Sycheva I O, Varich N L, Kaverin N V, Antonova T P, Chernos V I
Vopr Virusol. 1978 Sep-Oct(5):548-53.
Denaturated 3H-thymidine-labeled vaccinia virus DNA was hybridized with an excess of "late" virus-specific RNA isolated from virus-infected chick embryo cell cultures 8 hours postinfection. The percentage of DNA converted into DNA-RNA hybrid under these conditions never exceeded 50%. If the RNA preparation had been self-annealed prior to hybridization, the percentage was decreased slightly. On the other hand, if the self-annealed RNA had been treated with RN-ase and the double-stranded DNA-RNA hybrids had been denaturated, they became capable of converting into hybrids at least 68% of the labeled DNA. These data indicate that transcription of both DNA strands occurs in a large portion (over 68%) of vaccinia virus genome.
将变性的3H-胸腺嘧啶核苷标记的痘苗病毒DNA与从感染病毒8小时后的病毒感染鸡胚细胞培养物中分离出的过量“晚期”病毒特异性RNA进行杂交。在这些条件下转化为DNA-RNA杂交体的DNA百分比从未超过50%。如果RNA制剂在杂交前进行了自身退火，该百分比会略有下降。另一方面，如果对自身退火的RNA用核糖核酸酶处理，并且使双链DNA-RNA杂交体变性，它们能够将至少68%的标记DNA转化为杂交体。这些数据表明痘苗病毒基因组的很大一部分（超过68%）发生了两条DNA链的转录。
