Duesberg P H, Colby C
Proc Natl Acad Sci U S A. 1969 Sep;64(1):396-403. doi: 10.1073/pnas.64.1.396.
The virus-specific, RNase-resistant RNA appearing in vaccinia virus-infected cells was directly shown to be an RNA duplex. After its melting and subsequent banding on Cs(2)SO(4) or incubation with DNase, the RNA could be reannealed and then hybridized with vaccinia virus DNA.The double-stranded virus-specific RNA appears to exist in the cell in the form of heterogeneous partially double-stranded RNA's sedimenting between 9 and 22S. The kinetics of the appearance of the double-stranded RNA in the cell show a dependence on the multiplicity of infection and suggest that the double-stranded RNA is a "late" product of viral intracellular biosynthesis. The synthesis of the double-stranded RNA is inhibited by actinomycin D.
在牛痘病毒感染的细胞中出现的病毒特异性、抗核糖核酸酶的RNA被直接证明是一种RNA双链体。在其解链并随后在硫酸铯上进行区带离心或与脱氧核糖核酸酶一起温育后，该RNA可以重新退火，然后与牛痘病毒DNA杂交。双链病毒特异性RNA似乎以异质性部分双链RNA的形式存在于细胞中，沉降系数在9至22S之间。细胞中双链RNA出现的动力学表明其依赖于感染复数，并提示双链RNA是病毒细胞内生物合成的“晚期”产物。双链RNA的合成受到放线菌素D的抑制。
