痘苗病毒感染细胞中双链RNA的生物合成与结构
On the biosynthesis and structure of double-stranded RNA in vaccinia virus-infected cells.
作者信息
Duesberg P H, Colby C
出版信息
Proc Natl Acad Sci U S A. 1969 Sep;64(1):396-403. doi: 10.1073/pnas.64.1.396.
Abstract
The virus-specific, RNase-resistant RNA appearing in vaccinia virus-infected cells was directly shown to be an RNA duplex. After its melting and subsequent banding on Cs(2)SO(4) or incubation with DNase, the RNA could be reannealed and then hybridized with vaccinia virus DNA.The double-stranded virus-specific RNA appears to exist in the cell in the form of heterogeneous partially double-stranded RNA's sedimenting between 9 and 22S. The kinetics of the appearance of the double-stranded RNA in the cell show a dependence on the multiplicity of infection and suggest that the double-stranded RNA is a "late" product of viral intracellular biosynthesis. The synthesis of the double-stranded RNA is inhibited by actinomycin D.
摘要
在牛痘病毒感染的细胞中出现的病毒特异性、抗核糖核酸酶的RNA被直接证明是一种RNA双链体。在其解链并随后在硫酸铯上进行区带离心或与脱氧核糖核酸酶一起温育后，该RNA可以重新退火，然后与牛痘病毒DNA杂交。双链病毒特异性RNA似乎以异质性部分双链RNA的形式存在于细胞中，沉降系数在9至22S之间。细胞中双链RNA出现的动力学表明其依赖于感染复数，并提示双链RNA是病毒细胞内生物合成的“晚期”产物。双链RNA的合成受到放线菌素D的抑制。
相似文献
1
On the biosynthesis and structure of double-stranded RNA in vaccinia virus-infected cells.痘苗病毒感染细胞中双链RNA的生物合成与结构
Proc Natl Acad Sci U S A. 1969 Sep;64(1):396-403. doi: 10.1073/pnas.64.1.396.
2
Double-stranded RNA in vaccinia virus infected cells.痘苗病毒感染细胞中的双链RNA
Nature. 1969 Jun 7;222(5197):940-4. doi: 10.1038/222940a0.
3
Assembly of vaccinia virus particles from polypeptides made in the presence of rifampicin.在利福平存在的情况下由多肽组装痘苗病毒颗粒。
Virology. 1971 Jul;45(1):123-34. doi: 10.1016/0042-6822(71)90119-x.
4
Intermolecular duplexes formed from polyadenylylated vaccinia virus RNA.由聚腺苷酸化痘苗病毒RNA形成的分子间双链体。
J Virol. 1979 Apr;30(1):365-74. doi: 10.1128/JVI.30.1.365-374.1979.
5
Inhibition of protein synthesis by vaccinia virus. II. Studies on the role of virus-induced RNA synthesis.牛痘病毒对蛋白质合成的抑制作用。II. 关于病毒诱导的RNA合成作用的研究。
J Gen Virol. 1979 Sep;44(3):625-38. doi: 10.1099/0022-1317-44-3-625.
6
Virus interference by cellular double-stranded ribonucleic acid.细胞双链核糖核酸介导的病毒干扰
J Virol. 1971 Jun;7(6):697-706. doi: 10.1128/JVI.7.6.697-706.1971.
7
Evidence for an "early early" vaccinia virus-induced protein which causes a density change of infected L-M cells.一种由痘苗病毒“极早期”诱导产生的、可导致被感染的L-M细胞密度改变的蛋白质的证据。
J Virol. 1975 Jan;17(1):60-7. doi: 10.1128/JVI.17.1.60-67.1976.
8
9
Synthesis of vaccinia virus "early" and "late" messenger RNA in vitro with nucleoprotein structures isolated from infected cells.利用从感染细胞中分离出的核蛋白结构在体外合成牛痘病毒“早期”和“晚期”信使核糖核酸。
Virology. 1970 Oct;42(2):463-72. doi: 10.1016/0042-6822(70)90289-8.
10
[RNA transcripts of both strands of viral DNA in cells infected with vaccinia virus].[感染痘苗病毒的细胞中病毒DNA两条链的RNA转录本]
Vopr Virusol. 1978 Sep-Oct(5):548-53.
引用本文的文献
1
Host species-specific activity of the poxvirus PKR inhibitors E3 and K3 mediate host range function.痘病毒 PKR 抑制剂 E3 和 K3 的宿主物种特异性活性介导宿主范围功能。
J Virol. 2024 Nov 19;98(11):e0133124. doi: 10.1128/jvi.01331-24. Epub 2024 Oct 31.
2
Invading viral DNA triggers dsRNA synthesis by RNA polymerase II to activate antiviral RNA interference in Drosophila.入侵病毒的 DNA 通过 RNA 聚合酶 II 触发 dsRNA 的合成，从而激活果蝇中的抗病毒 RNA 干扰。
Cell Rep. 2022 Jun 21;39(12):110976. doi: 10.1016/j.celrep.2022.110976.
3
Vaccinia virus E3 prevents sensing of Z-RNA to block ZBP1-dependent necroptosis.痘苗病毒 E3 阻止 Z-RNA 的感应来阻断 ZBP1 依赖的坏死性凋亡。
Cell Host Microbe. 2021 Aug 11;29(8):1266-1276.e5. doi: 10.1016/j.chom.2021.05.009. Epub 2021 Jun 29.
4
Myxoma Virus-Encoded Host Range Protein M029: A Multifunctional Antagonist Targeting Multiple Host Antiviral and Innate Immune Pathways.黏液瘤病毒编码的宿主范围蛋白M029：一种靶向多种宿主抗病毒和固有免疫途径的多功能拮抗剂。
Vaccines (Basel). 2020 May 23;8(2):244. doi: 10.3390/vaccines8020244.
5
Ectromelia virus lacking the E3L ortholog is replication-defective and nonpathogenic but does induce protective immunity in a mouse strain susceptible to lethal mousepox.缺失 E3L 直系同源物的细小病毒是复制缺陷且非致病性的，但在易感染致死性鼠痘的小鼠品系中可诱导保护性免疫。
Virology. 2018 May;518:335-348. doi: 10.1016/j.virol.2018.03.016. Epub 2018 Mar 27.
6
Poxviruses Utilize Multiple Strategies to Inhibit Apoptosis.痘病毒利用多种策略抑制细胞凋亡。
Viruses. 2017 Aug 8;9(8):215. doi: 10.3390/v9080215.
7
Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells.与痘苗病毒相比，在BS - C - 1细胞中，鼠痘病毒积累的双链RNA较少。
Virology. 2017 Sep;509:98-111. doi: 10.1016/j.virol.2017.06.010.
8
Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus.猴痘病毒诱导合成的双链RNA比痘苗病毒少，并且比痘苗病毒更耐抗痘病毒药物IBT。
Virology. 2016 Oct;497:125-135. doi: 10.1016/j.virol.2016.07.016. Epub 2016 Jul 26.
9
Opposing Roles of Double-Stranded RNA Effector Pathways and Viral Defense Proteins Revealed with CRISPR-Cas9 Knockout Cell Lines and Vaccinia Virus Mutants.利用CRISPR-Cas9基因敲除细胞系和痘苗病毒突变体揭示双链RNA效应途径和病毒防御蛋白的相反作用
J Virol. 2016 Aug 12;90(17):7864-79. doi: 10.1128/JVI.00869-16. Print 2016 Sep 1.
10
The 2-5A system: modulation of viral and cellular processes through acceleration of RNA degradation.2-5A系统：通过加速RNA降解来调节病毒和细胞过程。
Pharmacol Ther. 1998 May;78(2):55-113. doi: 10.1016/s0163-7258(97)00167-8.
本文引用的文献
1
2
On the structure and replication of influenza virus.关于流感病毒的结构与复制
J Mol Biol. 1967 May 14;25(3):383-405. doi: 10.1016/0022-2836(67)90193-3.
3
Patterns of convergent and overlapping transcription within the b2 region of coliphage lambda.大肠杆菌噬菌体λ b2区域内的趋同转录和重叠转录模式。
Virology. 1969 Aug;38(4):614-26. doi: 10.1016/0042-6822(69)90181-0.
4
Double-stranded RNA in vaccinia virus infected cells.痘苗病毒感染细胞中的双链RNA
Nature. 1969 Jun 7;222(5197):940-4. doi: 10.1038/222940a0.
5
[Presence of a double chain ribonucleic acid in animal cells].[动物细胞中双链核糖核酸的存在]
C R Acad Hebd Seances Acad Sci D. 1968 Oct 21;267(17):1417-20.
6
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.一种用于检测固定在膜上的DNA与RNA杂交体的定量分析方法。
J Mol Biol. 1965 Jul;12(3):829-42. doi: 10.1016/s0022-2836(65)80331-x.
7
Recent progress in poxvirus research.痘病毒研究的最新进展。
Bacteriol Rev. 1968 Jun;32(2):127-37. doi: 10.1128/br.32.2.127-137.1968.
8
Ribonucleic acids from animal cells.来自动物细胞的核糖核酸。
Bacteriol Rev. 1968 Sep;32(3):262-90. doi: 10.1128/br.32.3.262-290.1968.
Zotero 插件