一种结合二硝基苯基配体的小鼠骨髓瘤蛋白的亲和位点标记。
Affinity site labeling of a mouse myeloma protein which binds dinitrophenyl ligands.
作者信息
Metzger H, Potter M
出版信息
Science. 1968 Dec 20;162(3860):1398-400. doi: 10.1126/science.162.3860.1398.
PMID:5699657
Abstract
A mouse myeloma protein of the immunoglobulin A class, which specifically binds dinitrophenyl ligands, has been successfully affinity labeled (site-directed labeling) with several diazonium reagents, leading to inactivation of the combining sites. The labeling reagents reacted only with tyrosine residues of light chain origin. The 7S subunit of the myeloma protein appears to contain only one reactive site.
摘要
一种免疫球蛋白A类的小鼠骨髓瘤蛋白，它能特异性结合二硝基苯基配体，已成功地用几种重氮试剂进行了亲和标记（定点标记），导致结合位点失活。标记试剂仅与轻链来源的酪氨酸残基反应。骨髓瘤蛋白的7S亚基似乎只含有一个反应位点。
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