Russell G K, Lyman H
Plant Physiol. 1968 Aug;43(8):1284-90. doi: 10.1104/pp.43.8.1284.
Four mutant strains of Euglena gracilis have been isolated after treatment of wild type cells with ultraviolet light or the chemical mutagen nitrosoguanidine. None of the mutants is capable of autotrophic growth or photosynthetic carbon dioxide fixation. The mutant strains contain normal amounts of the enzymes of the reductive pentose phosphate cycle and are qualitatively similar to the wild type in pigment composition, but are unable to carry out the Hill reaction (light induced reduction of 2,6-dichlorophenol indophenol). Isolated mutant plastids cannot photoreduce NADP with water as the electron donor but can carry out this reaction when the electron donating system is ascorbate and 2,6-dichlorophenol indophenol. Whole cells of the mutants show the light induced oxidation of cytochrome f by light reaction I but are unable to bring about cytochrome f reduction by light reaction II. The mutants appear to be blocked at or near light reaction II in the photosynthetic electron transport chain. The mutants may represent alterations of the chloroplast genome since the mutation isolation was carried out under conditions where chloroplast viability was severely impaired, but cell viability was unaffected.
在用紫外线或化学诱变剂亚硝基胍处理野生型细胞后，分离出了四株纤细裸藻突变株。这些突变株均不能进行自养生长或光合二氧化碳固定。突变株含有正常量的还原性戊糖磷酸循环的酶，色素组成在性质上与野生型相似，但不能进行希尔反应（光诱导的2,6 - 二氯酚靛酚还原）。分离出的突变质体不能以水作为电子供体光还原NADP，但当电子供体系统为抗坏血酸和2,6 - 二氯酚靛酚时能进行此反应。突变株的全细胞在光反应I中显示出光诱导的细胞色素f氧化，但在光反应II中不能使细胞色素f还原。这些突变株似乎在光合电子传递链的光反应II处或其附近受阻。由于突变分离是在叶绿体活力严重受损但细胞活力未受影响的条件下进行的，所以这些突变株可能代表叶绿体基因组的改变。
