Stahl F, Hubl W, Schnorr D, Dörner G
Endokrinologie. 1978 May;72(2):214-22.
A non-chromatographic competitive binding assay (CBA) using horse transcortin has been employed in the routine measurement of cortisol in plasma, urine and amniotic fluids. Comparing the values with those of a radioimmunoassay (RIA) or a fluorimetric method (FM) an excellent correlation between the three methods both in plasma and urine has been calculated in normal subjects and in patients with various endocrine disorders. In amniotic fluids, however, there were discrepancies between CBA and RIA. Whereas CBA showed no differences, RIA gave significantly higher values in amniotic fluids of female than of male fetuses. Elevated free plasma cortisol levels observed in patients with prostatic cancer after diethyl stilboestrol diphosphate therapy did not correlate with unconjugated urinary cortisol concentration as measured with CBA and FM. In newborns, a relatively high plasma level found 12 hours after birth was followed by a nadir on the 2nd and 3rd day of life and by an increase until levels of adults on the 5th day of life were reached.
一种使用马皮质转运蛋白的非色谱竞争性结合测定法（CBA）已用于血浆、尿液和羊水中皮质醇的常规测量。将这些值与放射免疫测定法（RIA）或荧光测定法（FM）的值进行比较，在正常受试者和患有各种内分泌疾病的患者中，已计算出这三种方法在血浆和尿液中的相关性都非常好。然而，在羊水中，CBA和RIA之间存在差异。CBA没有显示出差异，而RIA在女性胎儿的羊水中的值明显高于男性胎儿。在用二乙基己烯雌酚二磷酸治疗后，前列腺癌患者中观察到的游离血浆皮质醇水平升高与用CBA和FM测量的未结合尿皮质醇浓度无关。在新生儿中，出生后12小时发现相对较高的血浆水平，随后在出生后第2天和第3天降至最低点，然后升高，直到出生后第5天达到成人水平。
