温度对虹鳟鱼和南极鱼类伯氏南极鱼丙酮酸激酶催化及调节功能的影响。
The effect of temperature on catalytic and regulatory functions of pyruvate kinases of the rainbow trout and the antarctic fish Trematomus bernacchii.
作者信息
Somero G N, Hochachka P W
出版信息
Biochem J. 1968 Dec;110(3):395-400. doi: 10.1042/bj1100395.
Abstract
- The effects of temperature on the catalytic and regulatory properties of pyruvate kinases from the temperate-zone rainbow trout and the Antarctic fish Trematomus bernacchii were examined. 2. The K(m) value of pyruvate kinase for one of its two substrates, phosphoenolpyruvate, is temperature-dependent, and is lowest at temperatures that closely coincide with the habitat temperatures of the two fishes. 3. Two regulatory functions of pyruvate kinase, feedforward activation by fructose diphosphate and feedback inhibition by ATP, are temperature-independent. Enzyme-ADP interaction is also temperature-independent. 4. It is concluded that enzyme-substrate and enzyme-modulator interactions are important factors in short-term and in evolutionary adaptations by poikilotherms to changes in temperature. Though the K(m) for substrate may vary in apparently adaptive manners, the regulatory functions of an enzyme appear to be unchanged over the range of temperatures experienced by the organism in Nature.
摘要
- 研究了温度对温带虹鳟鱼和南极鱼类伯氏南极鱼丙酮酸激酶催化特性和调节特性的影响。2. 丙酮酸激酶对其两种底物之一磷酸烯醇丙酮酸的米氏常数（K(m)）与温度有关，在与这两种鱼类栖息地温度密切相符的温度下最低。3. 丙酮酸激酶的两种调节功能，即果糖二磷酸的前馈激活和ATP的反馈抑制，与温度无关。酶与ADP的相互作用也与温度无关。4. 得出结论，酶-底物和酶-调节剂相互作用是变温动物在短期内以及在进化过程中适应温度变化的重要因素。虽然底物的米氏常数（K(m)）可能以明显适应性的方式变化，但一种酶的调节功能在生物体在自然环境中经历的温度范围内似乎保持不变。
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