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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fitt P S, Fitt E A, Wille H

Biochem J. 1968 Dec;110(3):475-9. doi: 10.1042/bj1100475.

Trypsin digestion of Micrococcus lysodeikticus polynucleotide phosphorylase (nucleoside diphosphate-polynucleotide nucleotidyltransferase) causes a progressive increase in electrophoretic mobility in polyacrylamide gels of the single active degradation product. 2. A marked increase in primer requirement for CDP polymerization occurs before a more mobile product is formed. 3. alpha-Chymotrypsin digestion yields a product that separates into several active species on polyacrylamide-gel electrophoretograms. 4. No separation of ADP-and CDP-polymerization activities occurs during electrophoresis after either trypsin or alpha-chymotrypsin treatment.

用胰蛋白酶消化溶壁微球菌多核苷酸磷酸化酶（核苷二磷酸-多核苷酸核苷酸基转移酶），会使单一活性降解产物在聚丙烯酰胺凝胶中的电泳迁移率逐渐增加。2. 在形成迁移率更高的产物之前，CDP聚合反应的引物需求显著增加。3. α-胰凝乳蛋白酶消化产生的产物在聚丙烯酰胺凝胶电泳图谱上分离成几种活性成分。4. 用胰蛋白酶或α-胰凝乳蛋白酶处理后，电泳过程中ADP和CDP聚合活性均未分离。

Fitt P S, Fitt E A, Wille H

Biochem J. 1968 Dec;110(3):475-9. doi: 10.1042/bj1100475.

Trypsin digestion of Micrococcus lysodeikticus polynucleotide phosphorylase (nucleoside diphosphate-polynucleotide nucleotidyltransferase) causes a progressive increase in electrophoretic mobility in polyacrylamide gels of the single active degradation product. 2. A marked increase in primer requirement for CDP polymerization occurs before a more mobile product is formed. 3. alpha-Chymotrypsin digestion yields a product that separates into several active species on polyacrylamide-gel electrophoretograms. 4. No separation of ADP-and CDP-polymerization activities occurs during electrophoresis after either trypsin or alpha-chymotrypsin treatment.

用胰蛋白酶消化溶壁微球菌多核苷酸磷酸化酶（核苷二磷酸-多核苷酸核苷酸基转移酶），会使单一活性降解产物在聚丙烯酰胺凝胶中的电泳迁移率逐渐增加。2. 在形成迁移率更高的产物之前，CDP聚合反应的引物需求显著增加。3. α-胰凝乳蛋白酶消化产生的产物在聚丙烯酰胺凝胶电泳图谱上分离成几种活性成分。4. 用胰蛋白酶或α-胰凝乳蛋白酶处理后，电泳过程中ADP和CDP聚合活性均未分离。