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1
A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase.一项通过聚丙烯酰胺凝胶电泳研究蛋白水解作用对溶壁微球菌多核苷酸磷酸化酶影响的实验。
Biochem J. 1968 Dec;110(3):475-9. doi: 10.1042/bj1100475.
2
The effect of trypsin digestion on the activities of polynucleotide phosphorylase.胰蛋白酶消化对多核苷酸磷酸化酶活性的影响。
Biochem J. 1967 Oct;105(1):25-33. doi: 10.1042/bj1050025.
3
Preparatin, proteolysis and reversible oxidationof highly purified Azotobacter vinelandii polynucleotide phosphorylase.高纯度棕色固氮菌多核苷酸磷酸化酶的制备、蛋白水解及可逆氧化
Biochem J. 1970 Dec;120(4):753-61. doi: 10.1042/bj1200753.
4
POLYNUCLEOTIDE PHOSPHORYLASE OF MICROCOCCUS LYSODEIKTICUS. IV. GUANOSINE DIPHOSPHATE AS SUBSTRATE AND INHIBITOR.溶壁微球菌的多核苷酸磷酸化酶。IV. 二磷酸鸟苷作为底物和抑制剂。
J Biol Chem. 1964 Mar;239:893-901.
5
Polynucleotide phosphorylase of Micrococcus luteus (Micrococcus lysodeikticus). The effect of sulfhydryl compounds on the ability to catalyze the polymerization reaction.藤黄微球菌（溶壁微球菌）的多核苷酸磷酸化酶。巯基化合物对催化聚合反应能力的影响。
J Biol Chem. 1968 Oct 10;243(19):5094-100.
6
A convenient method for the preparation of primer-dependent polynucleotide phosphorylase from Micrococcus lysodeikticus.
Biochem Biophys Res Commun. 1967 Nov 17;29(3):356-61. doi: 10.1016/0006-291x(67)90462-7.
7
The processive degradation of individual polyribonucleotide chains. II. Micrococcus lysodeikticus polynucleotide phosphorylase.单个多聚核糖核苷酸链的持续降解。II. 溶壁微球菌多核苷酸磷酸化酶
J Biol Chem. 1968 Mar 10;243(5):923-7.
8
Polynucleotide phosphorylase of Micrococcus luteus. Studies on the polymerization reaction catalyzed by primer-dependent and primer-independent enzymes.藤黄微球菌的多核苷酸磷酸化酶。对依赖引物和不依赖引物的酶所催化的聚合反应的研究。
J Biol Chem. 1970 May 10;245(9):2414-22.
9
The preferential loss of the polylysine- or polyornithine-stimulated activity of Clostridium perfringens polynucleotide phosphorylase during proteolysis.产气荚膜梭菌多核苷酸磷酸化酶在蛋白水解过程中，其受聚赖氨酸或聚鸟氨酸刺激的活性优先丧失。
Biochem J. 1969 May;112(4):489-95. doi: 10.1042/bj1120489.
10
Polynucleotide phosphorylase of Micrococcus lysodeikticus. V. A modified preparative procedure.
J Biol Chem. 1966 Aug 10;241(15):3639-41.

引用本文的文献

1
Reversible oxidation of Azotobacter vinelandii polynucleotide phosphorylase.维涅兰德固氮菌多核苷酸磷酸化酶的可逆氧化作用
Biochem J. 1969 Apr;112(3):381-2. doi: 10.1042/bj1120381.
2
The influence of the size and nature of basic activators on Clostridium perfringens polynucleotide phosphorylase-catalysed polyadenylic acid synthesis.碱性激活剂的大小和性质对产气荚膜梭菌多核苷酸磷酸化酶催化的聚腺苷酸合成的影响。
Biochem J. 1969 May;112(4):497-503. doi: 10.1042/bj1120497.
3
The preferential loss of the polylysine- or polyornithine-stimulated activity of Clostridium perfringens polynucleotide phosphorylase during proteolysis.产气荚膜梭菌多核苷酸磷酸化酶在蛋白水解过程中，其受聚赖氨酸或聚鸟氨酸刺激的活性优先丧失。
Biochem J. 1969 May;112(4):489-95. doi: 10.1042/bj1120489.

本文引用的文献

1
SIMPLIFIED "DISC" (POLYACRYLAMIDE GEL) ELECTROPHORESIS.简易“圆盘”（聚丙烯酰胺凝胶）电泳
Ann N Y Acad Sci. 1964 Dec 28;121:428-36. doi: 10.1111/j.1749-6632.1964.tb14214.x.
2
The dependence of reactions catalyzed by polynucleotide phosphorylase on oligonucleotides.多核苷酸磷酸化酶催化的反应对寡核苷酸的依赖性。
J Biol Chem. 1962 Jan;237:182-9.
3
A convenient method for the preparation of primer-dependent polynucleotide phosphorylase from Micrococcus lysodeikticus.
Biochem Biophys Res Commun. 1967 Nov 17;29(3):356-61. doi: 10.1016/0006-291x(67)90462-7.
4
The effect of trypsin digestion on the activities of polynucleotide phosphorylase.胰蛋白酶消化对多核苷酸磷酸化酶活性的影响。
Biochem J. 1967 Oct;105(1):25-33. doi: 10.1042/bj1050025.
5
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离多种核糖核酸种类
Biochemistry. 1967 Jun;6(6):1818-27. doi: 10.1021/bi00858a033.
6
Disc electrophoresis of ribonucleic acid in polyacrylamide gels.核糖核酸在聚丙烯酰胺凝胶中的圆盘电泳。
Anal Biochem. 1965 Sep;12(3):452-71. doi: 10.1016/0003-2697(65)90212-5.
7
Some aspects of the use of "continuous" and "discontinuous" buffer systems in polyacrylamide gel electrophoresis.聚丙烯酰胺凝胶电泳中“连续”和“不连续”缓冲系统使用的一些方面。
Anal Biochem. 1965 May;11(2):219-23. doi: 10.1016/0003-2697(65)90008-4.
8
Polynucleotide phosphorylase: structure and mechanism of action.多核苷酸磷酸化酶：结构与作用机制
Biochem J. 1967 Jun;103(3):62P.

一项通过聚丙烯酰胺凝胶电泳研究蛋白水解作用对溶壁微球菌多核苷酸磷酸化酶影响的实验。

A study by polyacrylamide-gel electrophoresis of the effect of proteolysis on Micrococcus lysodeikticus polynucleotide phosphorylase.

作者信息

Fitt P S, Fitt E A, Wille H

出版信息

Biochem J. 1968 Dec;110(3):475-9. doi: 10.1042/bj1100475.

DOI:10.1042/bj1100475
PMID:5701678
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187375/
Abstract
  1. Trypsin digestion of Micrococcus lysodeikticus polynucleotide phosphorylase (nucleoside diphosphate-polynucleotide nucleotidyltransferase) causes a progressive increase in electrophoretic mobility in polyacrylamide gels of the single active degradation product. 2. A marked increase in primer requirement for CDP polymerization occurs before a more mobile product is formed. 3. alpha-Chymotrypsin digestion yields a product that separates into several active species on polyacrylamide-gel electrophoretograms. 4. No separation of ADP-and CDP-polymerization activities occurs during electrophoresis after either trypsin or alpha-chymotrypsin treatment.
摘要
  1. 用胰蛋白酶消化溶壁微球菌多核苷酸磷酸化酶（核苷二磷酸-多核苷酸核苷酸基转移酶），会使单一活性降解产物在聚丙烯酰胺凝胶中的电泳迁移率逐渐增加。2. 在形成迁移率更高的产物之前，CDP聚合反应的引物需求显著增加。3. α-胰凝乳蛋白酶消化产生的产物在聚丙烯酰胺凝胶电泳图谱上分离成几种活性成分。4. 用胰蛋白酶或α-胰凝乳蛋白酶处理后，电泳过程中ADP和CDP聚合活性均未分离。