Espejo R T, Canelo E S
J Virol. 1968 Nov;2(11):1235-40. doi: 10.1128/JVI.2.11.1235-1240.1968.
Phosphatidylethanolamine is the only phosphatide present in purified bacteriophage PM2 when obtained from bacteria grown and infected in minimal medium. The growth cycle of bacteriophage PM2 shows the basic general features described for virulent bacteriophages. PM2 infection of Pseudomonas BAL-31 causes a pronounced increase in deoxyribonucleic acid synthesis, but no detectable effect on the incorporation of phosphorus into lipid. When (32)P-prelabeled bacteria are infected with PM2, the specific activity of the phosphatide phosphorus in the virus is almost the same as that in the host bacteria labeled before infection. It is concluded that the viral phosphatide is not synthesized de novo after infection, but it probably is derived from preexisting cellular phosphatidylethanolamine.
当从在基本培养基中生长并感染的细菌中获得时,磷脂酰乙醇胺是纯化的噬菌体PM2中唯一存在的磷脂。噬菌体PM2的生长周期显示出烈性噬菌体所描述的基本一般特征。PM2感染铜绿假单胞菌BAL-31会导致脱氧核糖核酸合成显著增加,但对磷掺入脂质没有可检测到的影响。当用PM2感染预先用(32)P标记的细菌时,病毒中磷脂磷的比活性与感染前标记的宿主细菌中的几乎相同。得出的结论是,病毒磷脂在感染后不是重新合成的,而是可能源自预先存在的细胞磷脂酰乙醇胺。