Jargiello P
Somatic Cell Genet. 1978 Nov;4(6):647-60. doi: 10.1007/BF01543156.
Forty-three independent variants of the Novikoff hepatoma cell line have been isolated for their ability to use D-xylose, D-ribose, and/or L-arabinose as a sole carbon and energy source. The variants exhibited marked morphological changes and a loss or decrease of cloning efficiency in soft agar. The xylose and arabinose variants showed similar phenotypes while the ribose variants were a phenotypically heterogenous group. Two major classes of variants were found with regard to their specificity for pentoses: one class could grow on ribose, xylose, or arabinose, while the second class grew only on ribose. The lack of specificity for pentose use was correlated with the ability to use pentitols for growth. The frequency of pentose-utilizing clones was 5 X 10(-2) to 10(-3), and nitrosoguanidine treatment increased this frequency tenfold. Fluctuation analyses showed the appearance of pentose-utilizing variants to be a random event. Of the variants examined, 84% expressed a stable pentose phenotype, and of those, 6% were cold sensitive and 8% were temperature sensitive for pentose utilization. In addition to the suggested mutational basis for the pentose phenotype, two variants showed a large increase in chromosome number from 73 +/- 3 to 132 +/- 10.
已分离出43个诺维科夫肝癌细胞系的独立变体，它们能够将D-木糖、D-核糖和/或L-阿拉伯糖用作唯一的碳源和能源。这些变体表现出明显的形态变化，并且在软琼脂中的克隆效率有所丧失或降低。木糖和阿拉伯糖变体表现出相似的表型，而核糖变体是一个表型异质的群体。根据它们对戊糖的特异性，发现了两大类变体：一类可以在核糖、木糖或阿拉伯糖上生长，而另一类仅在核糖上生长。对戊糖利用缺乏特异性与利用戊糖醇生长的能力相关。利用戊糖的克隆频率为5×10^(-2)至10^(-3)，亚硝基胍处理使该频率增加了10倍。波动分析表明，利用戊糖的变体的出现是一个随机事件。在所检查的变体中，84%表现出稳定的戊糖表型，其中6%对戊糖利用是冷敏感的，8%是温度敏感的。除了所提出的戊糖表型的突变基础外，两个变体的染色体数目从73±3大幅增加到132±10。
