Riera C M, Brandt E J, Shulman S
Immunology. 1968 Dec;15(6):779-87.
Data presented in this report indicate that freezing of male accessory tissue in rabbits which have been previously sensitized by injection of saline extracts of rabbit male accessory glands material, elicits the formation almost exclusively of the IgG class of immunoglobulin and constitutes, therefore, a booster type of antigenic stimulation in these animals. It is suggested that the marked decrease in antibody activity shown by most of the iso-immunized rabbits following freezing of their male accessory tissue could be attributed to immune complex formation between the circulating antibodies and an autoantigen that may be released as a result of the cryo-immunization. An alternative explanation for the decrease in antibody activity is that the antibodies complex with the homologous autoantigen which is fixed but exposed in the freeze damaged tissue. The possibility that the antigenicity of antigen—antibody complexes may be a factor in the abrupt increase in antibody titre, from the minimum level at the 3rd or 4th day to a peak at the 7th or 8th day after cryo-immunization, is discussed.
本报告中呈现的数据表明，对先前通过注射兔雄性附属性腺物质的盐水提取物而致敏的兔子的雄性附属组织进行冷冻，几乎仅引发IgG类免疫球蛋白的形成，因此，在这些动物中构成一种加强型的抗原刺激。有人提出，大多数同种免疫的兔子在其雄性附属组织冷冻后抗体活性显著下降，可能归因于循环抗体与一种自身抗原之间形成免疫复合物，这种自身抗原可能是冷冻免疫的结果而释放出来的。抗体活性下降的另一种解释是，抗体与固定但暴露于冷冻损伤组织中的同源自身抗原形成复合物。还讨论了抗原-抗体复合物的抗原性可能是冷冻免疫后抗体滴度从第3天或第4天的最低水平突然增加到第7天或第8天达到峰值的一个因素的可能性。
