Yantorno C, Soanes W A, Gonder M J, Shulman S
Immunology. 1967 Apr;12(4):395-410.
Experimental cryosurgery has been found to result in the formation of circulating antibodies, directed against antigenic material of the tissue frozen. These antibodies were detected by passive haemagglutination and immunodiffusion. The tissue that was subjected to destructive freezing by means of this surgical procedure was the coagulating gland and seminal vesicle of rabbit. A probe carrying liquid nitrogen and a thermocouple assembly for measuring temperature changes was utilized. Control rabbits were manipulated in the same way but with no freezing. A large number of rabbits subjected to tissue freezing exhibited the development of a rapid antibody response, achieving a maximum titre in 7–10 days, followed by a decline. This antibody response has been compared to that seen following isoimmunization by injection. As an additional comparison, several rabbits were autoimmunized by injection of homogenate from their own accessory tissue; these rabbits also produced antibodies. It was found that the time sequence in the antibody production was quite similar for auto- and isoimmunization, but was quite distinctive for cryo-stimulation. By several means of evaluation, it was shown that the antibody response to the freezing of tissue was an autoantibody. It was not, however, an antibody against serum γ-globulin. The consequences of the freezing of tissue are thus seen to simulate the effects of incorporating adjuvant. This method of producing autoantibodies and investigating the nature of the response has been termed cryo-immunology.
实验性冷冻手术已被发现会导致循环抗体的形成，这些抗体针对冷冻组织的抗原物质。通过被动血凝反应和免疫扩散检测到了这些抗体。通过这种手术方法进行破坏性冷冻的组织是兔的凝固腺和精囊。使用了一个携带液氮的探头和一个用于测量温度变化的热电偶组件。对对照兔进行同样的操作，但不进行冷冻。大量接受组织冷冻的兔表现出快速的抗体反应，在7 - 10天内达到最高滴度，随后下降。这种抗体反应已与注射同种免疫后的反应进行了比较。作为进一步的比较，几只兔通过注射自身附属组织的匀浆进行自身免疫；这些兔也产生了抗体。结果发现，自身免疫和同种免疫在抗体产生的时间顺序上相当相似，但冷冻刺激则非常独特。通过几种评估方法表明，对组织冷冻的抗体反应是一种自身抗体。然而，它不是针对血清γ - 球蛋白的抗体。因此可以看出，组织冷冻的后果类似于加入佐剂的效果。这种产生自身抗体并研究反应性质的方法被称为冷冻免疫学。
