Shiwaku Y, Nagashima H, Duncalf R M, Duncalf D, Foldes F
Anesth Analg. 1979 Mar-Apr;58(2):93-8. doi: 10.1213/00000539-197903000-00007.
The influence of halothane, or naloxone, or halothane followed by naloxone was investigated in the in vitro myenteric plexus longitudinal muscle preparation of the guinea pig ileum. Halothane alone in 1.5 to 2.0% (v/v) concentration caused about 50% depression of the twitch and decreased both spontaneous acetylcholine (ACh) release (p less than 0.02) and volley output of ACh (p less than 0.02). Very high concentrations (greater than 1 micron) of naloxone caused a nonspecific, postsynaptic depression of the twitch. Higher than 100 nM concentrations of naloxone increased spontaneous ACh release, but had no effect on the volley output of ACh. Over a wide concentration range, from 15 nM to 3 micron, naloxone did not antagonize in the longitudinal muscle preparation the effects of halothane on any of the parameters investigated. These findings indicate that the sites of action of halothane and naloxone in this preparation are not identical.
在豚鼠回肠的体外肌间神经丛-纵肌标本中，研究了氟烷、纳洛酮或氟烷后接纳洛酮的影响。单独使用浓度为1.5%至2.0%（v/v）的氟烷会使抽搐幅度降低约50%，并减少乙酰胆碱（ACh）的自发释放（p<0.02）以及ACh的群集释放量（p<0.02）。极高浓度（大于1微摩尔）的纳洛酮会引起抽搐的非特异性突触后抑制。浓度高于100纳摩尔的纳洛酮会增加ACh的自发释放，但对ACh的群集释放量没有影响。在15纳摩尔至3微摩尔的广泛浓度范围内，纳洛酮在纵肌标本中不会拮抗氟烷对所研究的任何参数的影响。这些发现表明，在该标本中氟烷和纳洛酮的作用位点并不相同。
