Gurina L V, Petrikevich S B, Shul'ga A V, Rylkin S S
Mikrobiologiia. 1979 Mar-Apr;48(2):245-8.
Candida utilis IBFMY-405 was grown in a synthetic medium with glucose. Cells taken at the logarithmic phase of growth were studied. The cells were treated with the enzyme from Helix pomatia to prepare protoplasts which were separated by differential centrifugation into groups according to their size. Three protein fractions were isolated from each group and the amino acid composition of the proteins was determined. Proteins of the first fraction (cytoplasmic) prevailed in all of the protoplast groups while the content of proteins of the second fraction (intermediate or myosin-like) was the lowest. As the size of protoplasts increased, difference in the quantitative content of proteins from the first and second fractions became less pronounced. The content of proteins of the third fraction was 3.6 and 2.4 times higher in the protoplasts of the medium size than in the largest protoplasts. The amino acid composition of each protein fraction differed quantitatively and qualitatively in all of the protoplast groups.
产朊假丝酵母IBFMY - 405在含有葡萄糖的合成培养基中培养。研究了在对数生长期采集的细胞。用来自苹果螺的酶处理细胞以制备原生质体，通过差速离心根据其大小将原生质体分成不同组。从每组中分离出三个蛋白质组分，并测定了蛋白质的氨基酸组成。在所有原生质体组中，第一组分（细胞质）的蛋白质占主导，而第二组分（中间或肌球蛋白样）的蛋白质含量最低。随着原生质体大小的增加，第一和第二组分蛋白质的定量含量差异变得不那么明显。中等大小原生质体中第三组分蛋白质的含量分别是最大原生质体中含量的3.6倍和2.4倍。在所有原生质体组中，每个蛋白质组分的氨基酸组成在数量和质量上都有所不同。
