一种核酸结合蛋白的进一步表征。
Further characterisation of a nucleic acid binding protein.
作者信息
Banks G R, Spanos A
出版信息
Nucleic Acids Res. 1979 Mar;6(3):931-52. doi: 10.1093/nar/6.3.931.
A glycoprotein which binds to nucleic acids has now been purified from Ustilago maydis until free from detectable deoxyribonuclease activity. It binds to a variety of substrates and in doing so, makes them soluble in dilute trichloroacetic acid. Physical studies suggest that it forms a variety of aggregates under low ionic strength, but at high ionic strength the monomer consists of a single polypeptide chain. Preliminary experiments have detected this novel binding activity in bacterial, fungal and mammalian cells.
一种能与核酸结合的糖蛋白现已从玉米黑粉菌中纯化出来，直至检测不到脱氧核糖核酸酶活性。它能与多种底物结合，从而使这些底物可溶于稀三氯乙酸中。物理研究表明，在低离子强度下它会形成多种聚集体，但在高离子强度下，单体由一条单一的多肽链组成。初步实验已在细菌、真菌和哺乳动物细胞中检测到这种新型结合活性。
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