Girardier L, Seydoux J, Clausen T
J Gen Physiol. 1968 Dec;52(6):925-40. doi: 10.1085/jgp.52.6.925.
Membrane potentials were recorded in isolated segments of interscapular brown adipose tissue from rats. After equilibration at 29 degrees C in Krebs-Ringer bicarbonate buffer a mean value of -51 +/- 4 mv (SD) was found. This level could be maintained for up to 5 hr. The mean effective membrane resistance was 1.35 +/- 0.45 megohm. The membrane potential was a function of the extracellular potassium concentration. Ouabain (10(-6)-10(-3)M) and incubation in K-free buffer produced progressive depolarization. Epinephrine and norepinephrine in concentrations as low as 10(-8) g/ml produced a prompt depolarization. Cooling of the tissue and lowering of the oxygen tension caused a marked and reversible decrease in the membrane potential. In tissue obtained from cold-adapted rats, the membrane potential was considerably diminished. 6Assuming that the membrane potential is some function of the Na permeability of the plasma membrane it is suggested that an increase in the rate of active Na-K transport and ensuing ADP formation might contribute to the increase in respiration seen during exposure to thermogenic stimuli.
在大鼠肩胛间棕色脂肪组织的分离片段中记录膜电位。在29℃的 Krebs-Ringer 碳酸氢盐缓冲液中平衡后，发现平均值为 -51±4 mV（标准差）。该水平可维持长达5小时。平均有效膜电阻为1.35±0.45兆欧。膜电位是细胞外钾浓度的函数。哇巴因（10^(-6)-10^(-3)M）和在无钾缓冲液中孵育会导致逐渐去极化。浓度低至10^(-8) g/ml 的肾上腺素和去甲肾上腺素会引起迅速去极化。组织冷却和氧张力降低会导致膜电位显著且可逆地降低。在从冷适应大鼠获得的组织中，膜电位明显降低。假设膜电位是质膜钠通透性的某种函数，有人提出，主动钠钾转运速率的增加以及随之而来的 ADP 形成可能有助于解释在暴露于产热刺激期间观察到的呼吸增加。
