木瓜蛋白酶一级序列中活性位点组氨酸残基的位置。
The location of the active-site histidine residue in the primary sequence of papain.
作者信息
Husain S S, Lowe G
出版信息
Biochem J. 1968 Aug;108(5):861-6. doi: 10.1042/bj1080861.
Abstract
Papain that had been irreversibly inhibited with 1,3-dibromo[2-(14)C]acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and alpha-chymotrypsin the radioactive peptides were purified chromatographically. Their amino acid composition indicated that cysteine-25 and histidine-106 were cross-linked. Since cysteine-25 is known to be the active-site cysteine residue, histidine-106 must be the active-site histidine residue.
摘要
用1,3 - 二溴[2 - (14)C]丙酮不可逆抑制的木瓜蛋白酶用硼氢化钠还原，并用碘乙酸进行羧甲基化。用胰蛋白酶和α - 糜蛋白酶消化后，通过色谱法纯化放射性肽段。它们的氨基酸组成表明半胱氨酸 - 25和组氨酸 - 106发生了交联。由于已知半胱氨酸 - 25是活性位点的半胱氨酸残基，所以组氨酸 - 106必定是活性位点的组氨酸残基。
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本文引用的文献
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Chymotryptic digest of papain.木瓜蛋白酶的胰凝乳蛋白酶消化产物
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PAPAIN-CATALYSED HYDROLYSIS OF SOME HIPPURIC ESTERS. A NEW MECHANISM FOR PAPAIN-CATALYSED HYDROLYSIS.木瓜蛋白酶催化某些马尿酸酯的水解。木瓜蛋白酶催化水解的新机制。
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A STUDY OF SOME THIOL ESTER HYDROLYSES AS MODELS FOR THE DEACYLATION STEP OF PAPAIN-CATALYSED HYDROLYSES.一些硫醇酯水解反应作为木瓜蛋白酶催化水解脱酰基步骤模型的研究
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CURRENT STATUS OF THE STRUCTURE OF PAPAIN: THE LINEAR SEQUENCE, ACTIVE SULFHYDRYL GROUP, AND THE DISULFIDE BRIDGES.木瓜蛋白酶结构的当前状态：线性序列、活性巯基和二硫键。
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1276-83. doi: 10.1073/pnas.52.5.1276.
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ION-EXCHANGE CHROMATOGRAPHY OF AMINO-ACIDS: IMPROVEMENTS IN THE SINGLE COLUMN SYSTEM.氨基酸的离子交换色谱法：单柱系统的改进
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Structure of papain.木瓜蛋白酶的结构。
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