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[丸粒大小对冷冻保存的精母细胞呼吸活性和运动性的影响]

[Effect of pellet size on respiratory activity and motility of cryopreserved spermatocytes].

作者信息

Glander H J, Luppa D

出版信息

Dermatol Monatsschr. 1979 Apr;165(4):286-91.

PMID:572308
Abstract

The effect of different pellet volumes at freeze preservation in human semen was studied. It was measured the motility of spermatozoa during an incubation time of several hours at 37 degrees C and the alteration of oxygen consumption after adding rotenone, succinate, digitonine or 2,4-dinitrophenol. - The motility of cryopreserved spermatozoa does not show significant differences after preservation procedure. In respect of oxygen consumption the smallest differences from native material were obtained at a pellet volume of 0,2 to 0,3 cm3. Explanations for this behaviour are discussed.

摘要

研究了人类精液冷冻保存时不同颗粒体积的影响。在37摄氏度下孵育数小时期间测量精子活力,并在添加鱼藤酮、琥珀酸盐、洋地黄皂苷或2,4-二硝基苯酚后测量耗氧量的变化。 - 冷冻保存后的精子活力在保存程序后未显示出显著差异。就耗氧量而言,在颗粒体积为0.2至0.3立方厘米时,与天然材料的差异最小。讨论了这种行为的解释。

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