Neurath A R, Rubin B A, Stasny J T
J Virol. 1968 Oct;2(10):1086-95. doi: 10.1128/JVI.2.10.1086-1095.1968.
When crude preparations of adenovirus types 4 and 7 were treated with solutions of formamide, a complete or nearly complete inactivation of infectivity could be achieved without affecting the complement fixation titers of the same preparations. Studies on purified virions showed that the intersubunit bonds within the viral capsid were broken and single capsomers were released as the result of treatment by formamide. When subjected to the same treatment, hemagglutinins were sequentially converted into incomplete hemagglutinins (pentons), which were in turn converted into penton bases and fibers.
当用甲酰胺溶液处理4型和7型腺病毒的粗制制剂时，可实现感染性的完全或几乎完全灭活，而不影响相同制剂的补体结合滴度。对纯化病毒粒子的研究表明，病毒衣壳内的亚基间键被破坏，并且由于甲酰胺处理的结果，单个衣壳粒被释放出来。当进行相同处理时，血凝素依次转化为不完全血凝素（五邻体），而不完全血凝素又依次转化为五邻体基部和纤维。
