4型和7型腺病毒病毒粒子及血凝素中壳粒间键的甲酰胺裂解作用
Cleavage by formamide of intercapsomer bonds in adenovirus types 4 and 7 virions and hemagglutinins.
作者信息
Neurath A R, Rubin B A, Stasny J T
出版信息
J Virol. 1968 Oct;2(10):1086-95. doi: 10.1128/JVI.2.10.1086-1095.1968.
Abstract
When crude preparations of adenovirus types 4 and 7 were treated with solutions of formamide, a complete or nearly complete inactivation of infectivity could be achieved without affecting the complement fixation titers of the same preparations. Studies on purified virions showed that the intersubunit bonds within the viral capsid were broken and single capsomers were released as the result of treatment by formamide. When subjected to the same treatment, hemagglutinins were sequentially converted into incomplete hemagglutinins (pentons), which were in turn converted into penton bases and fibers.
摘要
当用甲酰胺溶液处理4型和7型腺病毒的粗制制剂时，可实现感染性的完全或几乎完全灭活，而不影响相同制剂的补体结合滴度。对纯化病毒粒子的研究表明，病毒衣壳内的亚基间键被破坏，并且由于甲酰胺处理的结果，单个衣壳粒被释放出来。当进行相同处理时，血凝素依次转化为不完全血凝素（五邻体），而不完全血凝素又依次转化为五邻体基部和纤维。
相似文献
1
Cleavage by formamide of intercapsomer bonds in adenovirus types 4 and 7 virions and hemagglutinins.4型和7型腺病毒病毒粒子及血凝素中壳粒间键的甲酰胺裂解作用
J Virol. 1968 Oct;2(10):1086-95. doi: 10.1128/JVI.2.10.1086-1095.1968.
2
The effect of nonaqueous solvents on the quaternary structure of viruses: disruption of adenovirus type 5 by formamide. (brief report).
Arch Gesamte Virusforsch. 1969;26(3):295-300. doi: 10.1007/BF01242382.
3
The fate of adenovirus type 7 deosyribonucleic acid during treatment of the virus with formamide.用甲酰胺处理7型腺病毒时其脱氧核糖核酸的命运
Arch Gesamte Virusforsch. 1970;32(2):116-24. doi: 10.1007/BF01249947.
4
Effect of formamide on the capsid morphology of adenovirus types 4 and 7.甲酰胺对4型和7型腺病毒衣壳形态的影响。
J Virol. 1968 Dec;2(12):1429-42. doi: 10.1128/JVI.2.12.1429-1442.1968.
5
Comparison between soluble components of adenovirus types 3 and 16 and of the intermediate strain 3-16 (the San Carlos agent).3型和16型腺病毒以及中间株3-16（圣卡洛斯因子）可溶性成分的比较。
Virology. 1968 Oct;36(2):201-11. doi: 10.1016/0042-6822(68)90137-2.
6
Interaction of p-chloromercuribenzoate with adenoviruses. Inactivation of haemagglutinins and degradation of virions of types 3, 4 and 7.对氯汞苯甲酸与腺病毒的相互作用。3型、4型和7型腺病毒血凝素的失活及病毒粒子的降解
J Gen Virol. 1968 Mar;2(2):215-29. doi: 10.1099/0022-1317-2-2-215.
7
Comparative studies on the soluble components of adenovirus types 9 and 15 and the intermediate strain 9-15.9型和15型腺病毒以及中间株9-15可溶性成分的比较研究。
J Virol. 1968 Oct;2(10):1200-10. doi: 10.1128/JVI.2.10.1200-1210.1968.
8
Separation of adenovirus type 4 components.4型腺病毒成分的分离
Arch Gesamte Virusforsch. 1967;21(3):366-82. doi: 10.1007/BF01241737.
9
[Morphology of bovine adenovirus type I].
Vet Med Nauki. 1977;14(4):11-6.
10
Biophysical comparison of two canine adenoviruses.两种犬腺病毒的生物物理比较
J Virol. 1970 Apr;5(4):507-12. doi: 10.1128/JVI.5.4.507-512.1970.
引用本文的文献
1
The effect of nonaqueous solvents on the quaternary structure of viruses: disruption of adenovirus type 5 by formamide. (brief report).
Arch Gesamte Virusforsch. 1969;26(3):295-300. doi: 10.1007/BF01242382.
2
Effect of formamide on the capsid morphology of adenovirus types 4 and 7.甲酰胺对4型和7型腺病毒衣壳形态的影响。
J Virol. 1968 Dec;2(12):1429-42. doi: 10.1128/JVI.2.12.1429-1442.1968.
3
The fate of adenovirus type 7 deosyribonucleic acid during treatment of the virus with formamide.用甲酰胺处理7型腺病毒时其脱氧核糖核酸的命运
Arch Gesamte Virusforsch. 1970;32(2):116-24. doi: 10.1007/BF01249947.
4
Disruption of adenovirus type 7 by lithium iodide resulting in the release of viral deoxyribonucleic acid.碘化锂对7型腺病毒的破坏导致病毒脱氧核糖核酸的释放。
J Virol. 1970 Feb;5(2):173-8. doi: 10.1128/JVI.5.2.173-178.1970.
本文引用的文献
1
Architecture of the Adenovirus Capsid.腺病毒衣壳的结构
J Bacteriol. 1965 Jul;90(1):254-61. doi: 10.1128/jb.90.1.254-261.1965.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
POLAR STRIPPING OF PROTEIN SUBUNITS FROM TOBACCO MOSAIC VIRUS.从烟草花叶病毒中对蛋白质亚基进行极性剥离
Virology. 1965 Apr;25:502-7. doi: 10.1016/0042-6822(65)90077-2.
4
[ANALYSIS OF THE SERUM FRACTIONS OBTAINED WITH RIVANOL].
Acta Med Scand. 1965 Apr;177:427-31.
5
ALKALINE DEGRADATION OF TURNIP YELLOW MOSAIC VIRUS. I. THE CONTROLLED FORMATION OF EMPTY PROTEIN SHELLS.芜菁黄花叶病毒的碱性降解。I. 空蛋白壳的可控形成。
Biochemistry. 1964 Apr;3:486-93. doi: 10.1021/bi00892a004.
6
ASSEMBLY AND STABILITY OF THE TOBACCO MOSAIC VIRUS PARTICLE.烟草花叶病毒粒子的组装与稳定性
Adv Protein Chem. 1963;18:37-121. doi: 10.1016/s0065-3233(08)60268-5.
7
RECENT STUDIES ON THE STRUCTURE OF TOBACCO MOSAIC VIRUS.烟草花叶病毒结构的近期研究
Adv Protein Chem. 1963;18:1-35. doi: 10.1016/s0065-3233(08)60267-3.
8
THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE.尿素、甲酰胺和二醇对DEAE - 纤维素多核苷酸离子交换色谱中二级结合力的影响
Biochemistry. 1963 Jul-Aug;2:697-702. doi: 10.1021/bi00904a013.
9
STRUCTURE OF TYPE 5 ADENOVIRUS. I. ANTIGENIC RELATIONSHIP OF VIRUS-STRUCTURAL PROTEINS TO VIRUS-SPECIFIC SOLUBLE ANTIGENS FROM INFECTED CELLS.5型腺病毒的结构。I.病毒结构蛋白与感染细胞中病毒特异性可溶性抗原的抗原关系。
J Exp Med. 1963 Aug 1;118(2):295-306. doi: 10.1084/jem.118.2.295.
10
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
Zotero 插件