Stasny J T, Neurath A R, Rubin B A
J Virol. 1968 Dec;2(12):1429-42. doi: 10.1128/JVI.2.12.1429-1442.1968.
Treatment of adenovirus types 4 and 7 with formamide disrupted the virions, degrading the capsids into predominantly single capsomers. As shown by electron microscopic observation, disruption proceeded in the following sequence: (i) reduction of the electron density of the virions, suggesting release of an internal component; (ii) progressive cleavage of the capsid into two or more segments and the formation (type 7 only) of capsomer "sheets"; (iii) final cleavage of the capsid into single or groups of a few capsomers. The sequence appeared similar for both adenoviruses; for both types, the rate and extent of disruption were dependent on the formamide concentration, but type 7 was more easily disrupted than type 4 by short-term (5 to 10 sec) treatment at the low (10%) concentration. At 30% formamide, the intercapsomer bonds of either type were fully cleaved, and the capsids were completely degraded into predominantly single capsomers. Pretreatment with formaldehyde did not prevent this degradation. Under suitable conditions, virus-derived remnants can be observed among the breakdown products. These remnants have been shorn of capsomers and presumably represent intact internal nucleoprotein.
用甲酰胺处理4型和7型腺病毒会破坏病毒粒子，将衣壳降解为主要是单个壳粒。如电子显微镜观察所示，破坏过程按以下顺序进行：(i) 病毒粒子电子密度降低，表明内部成分释放；(ii) 衣壳逐渐裂解为两个或更多片段，并形成（仅7型）壳粒“片层”；(iii) 衣壳最终裂解为单个或几个壳粒组成的组。两种腺病毒的过程似乎相似；对于两种类型，破坏的速率和程度取决于甲酰胺浓度，但在低（10%）浓度下短期（5至10秒）处理时，7型比4型更容易被破坏。在30%甲酰胺浓度下，两种类型的壳粒间键均被完全裂解，衣壳完全降解为主要是单个壳粒。用甲醛预处理并不能阻止这种降解。在合适条件下，在分解产物中可观察到病毒衍生的残余物。这些残余物已去除壳粒，推测代表完整的内部核蛋白。
