Ferguson D J, Birch-Andersen A, Siim J C, Hutchison W M
Acta Pathol Microbiol Scand B. 1979 Jun;87B(3):183-90. doi: 10.1111/j.1699-0463.1979.tb02423.x.
The ultrastructural changes observed during sporocyst formation and the structure of the sporocyst wall was examined in oocysts which had been allowed to sporulate for between 12 and 48 hours at 27 degrees C. As the spherical sporoblast developed into the sporocyst the cytoplasmic mass became ellipsoidal in shape although no change was noted in the organelle compliment, which cosisted of two nuclei plus a number of polysaccharide granules, lipid globules, mitochondria, Golgi bodies, and some rough endoplasmic reticulum. The sporocyst wall consisted of a thin outer layer (15-20 nm) which was formed from two limiting membranes of the sporoblast and an inner layer (40-50 nm) which was comprised of four curved plates. This inner layer was formed under the outer layer and, although no specific cytoplasmic organelle disappeared with its formation, some unit membranes were observed close to the plasmalemma during its formation. Each curved plate has a marginal swelling and an interposing strip of material is present between the margins of adjacent plates. The plates are joined to the interposing strip by a thin band of osmiophilic material. In oblique and tangential sections through the plates two types of cross banding were observed which differed in periodicity.
在27摄氏度下让卵囊孢子化12至48小时后，对孢子囊形成过程中观察到的超微结构变化以及孢子囊壁的结构进行了检查。随着球形的孢子母细胞发育成孢子囊，细胞质团呈椭圆形，尽管细胞器组成没有变化，其细胞器包括两个细胞核以及一些多糖颗粒、脂质球、线粒体、高尔基体和一些粗面内质网。孢子囊壁由一层薄的外层（15 - 20纳米）和一层内层（40 - 50纳米）组成，外层由孢子母细胞的两个限制膜形成，内层由四个弯曲的板组成。这层内层在外层之下形成，虽然其形成过程中没有特定的细胞质细胞器消失，但在其形成过程中观察到一些单位膜靠近质膜。每个弯曲的板都有一个边缘肿胀，相邻板的边缘之间有一条插入的物质带。这些板通过一条嗜锇物质的细带与插入带相连。在穿过这些板的斜向和切向切片中，观察到两种类型的交叉条纹，其周期不同。
