一种能够结合多种特异性凝集素的多功能免疫吸附剂及其用于细胞群体分离的用途。
A versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations.
作者信息
Pereira M E, Kabat E A
出版信息
J Cell Biol. 1979 Jul;82(1):185-94. doi: 10.1083/jcb.82.1.185.
A procedure for cell fractionation using lectin-affinity chromatography is described. It consists of a single affinity adsorbent, hog gastric mucin blood group A+H substance covalently coupled to Sephadex or Sepharose, to which lectins of various specificities can bind. The complex formed, lectin in equilibrium hog A+H substance-Sephadex, then serves as an affinity probe for isolating and fractionating cells. The lectins from Ulex europaeus, Lotus tetragonolobus, Helix pomatia, Dolichos biflorus, and Phaseolus lunatus were used with the same blood group substance as adsorbent. The affinity columns retained erythrocytes with blood group specificity for the adsorbed lectin and thus fractionate cells in mixtures. Cells as well as lectins are eluted by specific sugar inhibitors. Mixtures of two kinds of cells can be separated when the proportion of the adsorbed cells is not too low.
本文描述了一种使用凝集素亲和色谱法进行细胞分级分离的方法。它由一种单一的亲和吸附剂组成，即猪胃粘蛋白血型A+H物质与共价偶联到葡聚糖凝胶或琼脂糖上，各种特异性的凝集素可以结合到该物质上。形成的复合物，即处于平衡状态的凝集素-猪A+H物质-葡聚糖凝胶，随后用作分离和分级细胞的亲和探针。使用来自欧洲荆豆、四角百脉根、苹果蜗牛、双花扁豆和菜豆的凝集素与相同的血型物质作为吸附剂。亲和柱保留了对吸附凝集素有血型特异性的红细胞，从而在混合物中对细胞进行分级分离。细胞和凝集素都可以用特定的糖抑制剂洗脱。当吸附细胞的比例不太低时，两种细胞的混合物可以被分离。
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