Zalewski P D, Forbes I J, Uhlenbruck G, Valente L
Clin Exp Immunol. 1981 May;44(2):304-14.
Binding of four blood group A-specific lectins, from Helix pomatia (HP), lucorum (HL), Cepaea nemoralis (CN) and Dolichos biflorus (DB) to human lymphocytes was determined. Use was made of lectins attached to latex particles, as convenient probes to detect binding of lectins to individual cells, Specificity of lectin-latex probes was demonstrated by comparison with agglutination and fluorescent lectin binding; experiments with untreated and enzyme-treated human and animal erythrocytes confirmed the specificity of the lectin-latex beads as did inhibition in the presence of N-acetyl-galactosamine, the immunodominant sugar of blood group A substance. The binding of HP to T cells and a proportion of B, and non-B, non-T cells is confirmed. Binding to B cells in chronic lymphocytic leukaemia (CLL) is also confirmed. HP-latex had the same specificity as soluble HP, but binding did not require pretreatment of lymphocytes with neuraminidase. HL-latex had the same specificity for erythrocytes, normal lymphocyte subpopulations and CLL lymphocytes as HP, but the binding was weaker and required pretreatment of the lymphocytes with neuraminidase to demonstrate it. Contrary to expectation, CN and DB bound to B lymphocytes and did not bind to T lymphocytes. CN binding was almost completely restricted to B cells, whereas DB bound to part of the non-B, non-T populations as well as to a substantial proportion of B cells. Neither CN nor DB labelled malignant T cells. Both CN and DB bound to B cells in CLL. As with HP, pretreatment of lymphocytes with neuraminidase increased the accessibility of receptors, but was not essential to demonstrate labelling with CN-, and DB-latex preparations. Binding of each lectin to lymphocytes was independent of ABO blood group of donor suggesting that ABO antigens may be expressed poorly, if at all, on lymphocytes. Binding is probably due to additional specificities possessed by these lectins. Protease treatment destroyed CN- and DB-binding sites but not HP receptors suggesting that at least two types of binding site are involved.
测定了来自苹果螺（HP）、福寿螺（HL）、黄蛞蝓（CN）和双花扁豆（DB）的四种血型A特异性凝集素与人淋巴细胞的结合情况。使用附着在乳胶颗粒上的凝集素作为方便的探针来检测凝集素与单个细胞的结合，通过与凝集反应和荧光凝集素结合进行比较，证明了凝集素 - 乳胶探针的特异性；用未处理和酶处理的人和动物红细胞进行的实验以及在N - 乙酰半乳糖胺（血型A物质的免疫显性糖）存在下的抑制实验证实了凝集素 - 乳胶珠的特异性。证实了HP与T细胞以及一部分B细胞和非B非T细胞的结合。也证实了其与慢性淋巴细胞白血病（CLL）中B细胞的结合。HP - 乳胶与可溶性HP具有相同的特异性，但结合不需要用神经氨酸酶对淋巴细胞进行预处理。HL - 乳胶对红细胞、正常淋巴细胞亚群和CLL淋巴细胞具有与HP相同的特异性，但结合较弱，需要用神经氨酸酶对淋巴细胞进行预处理才能显示出来。与预期相反，CN和DB与B淋巴细胞结合，而不与T淋巴细胞结合。CN的结合几乎完全局限于B细胞，而DB与部分非B非T群体以及相当一部分B细胞结合。CN和DB都不标记恶性T细胞。CN和DB都与CLL中的B细胞结合。与HP一样，用神经氨酸酶对淋巴细胞进行预处理增加了受体的可及性，但对于用CN - 和DB - 乳胶制剂进行标记来说并非必不可少。每种凝集素与淋巴细胞的结合与供体的ABO血型无关，这表明ABO抗原在淋巴细胞上可能表达不佳，甚至根本不表达。结合可能是由于这些凝集素具有额外的特异性。蛋白酶处理破坏了CN和DB的结合位点，但没有破坏HP受体，这表明至少涉及两种类型的结合位点。
