Hasholt L
J Med Genet. 1979 Aug;16(4):267-9. doi: 10.1136/jmg.16.4.267.
An abnormal cell population, 45,X, appeared in 3 of 4 cell lines established from an amniotic fluid specimen obtained from a normal mid-trimester pregnancy. Two of the cell lines were subjected to repeated chromosome analyses until VII passage. The abnormal cells were suppressed after repeated trypsinisations; simultaneously, fibroblast-like cells outgrew the cultures, which were previously predominated by epithelial-like cells. Polyploidy was found in 0 to 12% of the cells, the highest level existing in the early passages. The question of whether chromosomally abnormal cells present in primary cultures and the early subcultures reflect the karyotype of the fetus is discussed.
从一名正常妊娠中期孕妇的羊水标本中建立的4个细胞系中，有3个出现了异常细胞群45,X。其中2个细胞系进行了反复的染色体分析，直至第VII代传代。经过反复胰蛋白酶消化后，异常细胞受到抑制；与此同时，成纤维细胞样细胞在培养物中生长超过了之前以上皮样细胞为主的细胞。在0%至12%的细胞中发现了多倍体，最高水平出现在早期传代中。文中讨论了原代培养和早期传代培养中存在的染色体异常细胞是否反映胎儿核型的问题。
