Olsen R H, Metcalf E S, Brandt C
J Virol. 1968 Dec;2(12):1393-9. doi: 10.1128/JVI.2.12.1393-1399.1968.
Restriction of Pseudomonas bacteriophage CB3 growth on some Pseudomonas aeruginosa hosts was studied. On restricting hosts, growth of this phage was severely inhibited below 32 C and hence was temperature-sensitive. Investigation of this phenomenon revealed that restricting hosts were not killed as a consequence of their infection under nonpermissive conditions. The ability of some hosts to restrict showed segregation in sexual crosses between restricting and nonrestricting hosts. However, the pattern of restriction among various hosts differed with the phage in question when other phages were compared with CB3. Temperature-shift experiments indicated that blockage of an early event in the phage lytic cycle occurred when restricting conditions were imposed on cells infected with CB3. This blockage could be eliminated by holding at permissive conditions until the cold-sensitive step was bypassed or by pulsing restricting cells for 5 min at 37 C.
研究了铜绿假单胞菌噬菌体CB3在某些铜绿假单胞菌宿主上的生长限制情况。在限制型宿主上，该噬菌体在32℃以下生长受到严重抑制，因此具有温度敏感性。对这一现象的研究表明，限制型宿主在非允许条件下感染后不会被杀死。一些宿主的限制能力在限制型宿主与非限制型宿主的有性杂交中表现出分离现象。然而，当将其他噬菌体与CB3进行比较时，不同宿主之间的限制模式因所讨论的噬菌体而异。温度转换实验表明，当对感染CB3的细胞施加限制条件时，噬菌体裂解周期的早期事件会被阻断。通过在允许条件下保持直至绕过冷敏感步骤，或者通过在37℃下对限制型细胞进行5分钟的脉冲处理，可以消除这种阻断。
