肌动蛋白微丝和刷毛外套参与间隙连接膜内化的证据。
Evidence for the participation of actin microfilaments and bristle coats in the internalization of gap junction membrane.
作者信息
Larsen W J, Tung H N, Murray S A, Swenson C A
出版信息
J Cell Biol. 1979 Dec;83(3):576-87. doi: 10.1083/jcb.83.3.576.
Abstract
Thin sections of rabbit granulosa, human SW-13 adrenal cortical adenocarcinoma, and mouse B-16 melanoma cells revealed an apparent single-layered basket of 4- to 7-nm filaments surrounding cytoplasmic gap junction vesicles. This interpretation was based upon apparent longitudinal, cross, and en face sections of structures surrounding the vesicle profiles in tissue treated with tannic acid-glutaraldehyde. In granulosa cells incubated with the S-1 fragment of heavy meromyosin, arrowhead-decorated filaments were observed at the periphery of the gap junction vesicles, suggesting that these filaments contained actin. In addition, we found that small gap junctional blebs and vesicles at the cell surface were coated with short electron-dense bristles similar in appearance to the cloathrin-containing coat of coated vesicles of nonjunctional membrane. The role of these and other cytoskeletal elements in the possible endocytosis of gap junction membrane is discussed.
摘要
兔颗粒细胞、人SW - 13肾上腺皮质腺癌以及小鼠B - 16黑色素瘤细胞的薄切片显示，在细胞质间隙连接小泡周围有一个明显的由4至7纳米细丝构成的单层篮状结构。这一解释基于用单宁酸 - 戊二醛处理过的组织中围绕小泡轮廓的结构的明显纵向、横向和正面切片。在用重酶解肌球蛋白的S - 1片段孵育的颗粒细胞中，在间隙连接小泡的周边观察到箭头状装饰的细丝，这表明这些细丝含有肌动蛋白。此外，我们发现细胞表面的小间隙连接小泡和小泡被短的电子致密刷毛覆盖，其外观类似于非连接膜包被小泡的含网格蛋白的包被。讨论了这些以及其他细胞骨架成分在间隙连接膜可能的内吞作用中的作用。
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