Bentley D, Keshishian H, Shankland M, Toroian-Raymond A
J Embryol Exp Morphol. 1979 Dec;54:47-74.
During development of the grasshopper embryo, it is feasible to examine the structure, pharmacology, and physiology of uniquely identified cells. These experiments require a fast, accurate staging system suitable for live embryos. We present a system comprising (1) subdivision of embryogenesis into equal periods, (2) expression of stage in percent of complete embryogenesis time, (3) characterization of stages by light micrographs (and descriptive test), and (4) illustration of stages at the egg, embryo, and limb levels of resolution. Advantages of a percent-system include communicability, flexibility in temporal resolution, accurate assignment of elapsed time in developmental processes, and uniform coverage of the period of embryogenesis. The stages described are at 5% intervals with an estimated error of +/- 1%.
在蝗虫胚胎发育过程中，检查唯一识别细胞的结构、药理学和生理学是可行的。这些实验需要一个适用于活体胚胎的快速、准确的分期系统。我们提出了一个系统，该系统包括：（1）将胚胎发育细分为相等的时期；（2）以完成胚胎发育时间的百分比表示阶段；（3）通过光学显微镜照片（和描述性测试）对阶段进行表征；（4）在卵、胚胎和肢体分辨率水平上展示阶段。百分比系统的优点包括可交流性、时间分辨率的灵活性、在发育过程中对经过时间的准确分配以及胚胎发育时期的均匀覆盖。所描述的阶段以5%的间隔划分，估计误差为+/- 1%。
