Klenikova V A, Pevzner L Z
Microsc Acta. 1979 Nov;82(3):207-14.
Cytospectrophotometry of agar films soaked with serum albumin and of nervous tissue cells has shown that the histochemical colour reaction of proteins with Amido Black 10 B obeys Beer's law and that the spectral characteristics of this reaction are not significantly changed after fixation and histological procedure of nervous tissue. In cats, after in situ electrostimulation, and in rats, after anticipation stress, quantitative changes in the total protein content per cell were revealed in nerve and glial cells of cat sympathetic ganglion and rat spinal cord with the aid of cytospectrophotometry of Amido Black-stained sections. Staining with Amido Black 10B is recommended for cytospectrophotometric determinations of total protein and of separate protein fractions in nervous system cells.
对用血清白蛋白浸泡的琼脂膜以及神经组织细胞进行细胞分光光度测定表明，蛋白质与氨基黑10B的组织化学反应遵循比尔定律，并且在神经组织固定和组织学处理后，该反应的光谱特征没有明显变化。在猫身上，原位电刺激后，以及在大鼠身上，预期应激后，借助对氨基黑染色切片的细胞分光光度测定，在猫交感神经节和大鼠脊髓的神经和神经胶质细胞中发现了每个细胞总蛋白含量的定量变化。建议用氨基黑10B染色进行细胞分光光度法测定神经细胞中的总蛋白和单独的蛋白组分。
